Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry.

Andrew Palmer; R Griffiths; I Styles; Elzbieta Claridge; A Calcagni; J Bunch; Iain Styles

doi:10.1002/jms.2049

Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry.

Andrew Palmer, R Griffiths, I Styles, Elzbieta Claridge, A Calcagni, J Bunch, Iain Styles

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23 Citations (Scopus)

Abstract

Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-µm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections. Copyright © 2012 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	237-41
Number of pages	5
Journal	Journal of mass spectrometry : JMS
Volume	47
Issue number	2
DOIs	https://doi.org/10.1002/jms.2049
Publication status	Published - 1 Feb 2012

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10.1002/jms.2049

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abstract = "Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-µm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections. Copyright {\textcopyright} 2012 John Wiley & Sons, Ltd.",

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AU - Calcagni, A

AU - Bunch, J

AU - Styles, Iain

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AB - Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-µm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections. Copyright © 2012 John Wiley & Sons, Ltd.

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Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry.

Abstract

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