TY - JOUR
T1 - Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes
AU - Sundstrom, H
AU - Wallberg, F
AU - Ledung, E
AU - Norrman, B
AU - Hewitt, Christopher
AU - Enfors, SO
PY - 2004/1/1
Y1 - 2004/1/1
N2 - In Escherichia coli fermentation processes, a drastic drop in viable cell count as measured by the number of colony forming units per ml (c.f.u. ml(-1)) is often observed. This phenomenon was investigated in a process for the production of the recombinant fusion protein, promegapoietin (PMP). After induction, the number of c.f.u. ml(-1) dropped to approximately 10% of its maximum though the biomass concentration continued to increase. Flow cytometric analysis of viability and intracellular concentration of PMP showed that almost all cells were alive and contributed to the production. Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death.
AB - In Escherichia coli fermentation processes, a drastic drop in viable cell count as measured by the number of colony forming units per ml (c.f.u. ml(-1)) is often observed. This phenomenon was investigated in a process for the production of the recombinant fusion protein, promegapoietin (PMP). After induction, the number of c.f.u. ml(-1) dropped to approximately 10% of its maximum though the biomass concentration continued to increase. Flow cytometric analysis of viability and intracellular concentration of PMP showed that almost all cells were alive and contributed to the production. Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death.
KW - viability
KW - fed-batch fermentation
KW - bacterial cell division
KW - Escherichia coli
KW - flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=21644447627&partnerID=8YFLogxK
U2 - 10.1023/B:BILE.0000044458.29147.75
DO - 10.1023/B:BILE.0000044458.29147.75
M3 - Article
C2 - 15604793
SN - 1573-6776
VL - 26
SP - 1533
EP - 1539
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 19
ER -