TY - JOUR
T1 - Reorientation of microfluidic channel enables versatile dielectrophoretic platforms for cell manipulations
AU - Tang, S.-Y.
AU - Zhang, W.
AU - Yi, P.
AU - Baratchi, S.
AU - Kalantar-zadeh, K.
AU - Khoshmanesh, K.
PY - 2013
Y1 - 2013
N2 - Dielectrophoresis is a versatile tool for the sorting, immobilization, and characterization of cells in microfluidic systems. The performance of dielectrophoretic systems strongly relies on the configuration of microelectrodes, which produce a nonuniform electric field. However, once fabricated, the microelectrodes cannot be reconfigured to change the characteristics of the system. Here, we show that the reorientation of the microfluidic channel with respect to the microelectrodes can be readily utilized to alter the characteristics of the system. This enables us to change the location and density of immobilized viable cells across the channel, release viable cells along customized numbers of streams within the channel, change the deflection pattern of nonviable cells along the channel, and improve the sorting of viable and nonviable cells in terms of flow throughput and efficiency of the system. We demonstrate that the reorientation of the microfluidic channel is an effective tool to create versatile dielectrophoretic platforms using the same microelectrode design.
AB - Dielectrophoresis is a versatile tool for the sorting, immobilization, and characterization of cells in microfluidic systems. The performance of dielectrophoretic systems strongly relies on the configuration of microelectrodes, which produce a nonuniform electric field. However, once fabricated, the microelectrodes cannot be reconfigured to change the characteristics of the system. Here, we show that the reorientation of the microfluidic channel with respect to the microelectrodes can be readily utilized to alter the characteristics of the system. This enables us to change the location and density of immobilized viable cells across the channel, release viable cells along customized numbers of streams within the channel, change the deflection pattern of nonviable cells along the channel, and improve the sorting of viable and nonviable cells in terms of flow throughput and efficiency of the system. We demonstrate that the reorientation of the microfluidic channel is an effective tool to create versatile dielectrophoretic platforms using the same microelectrode design.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84877838774&partnerID=MN8TOARS
U2 - 10.1002/elps.201200659
DO - 10.1002/elps.201200659
M3 - Article
SN - 0173-0835
VL - 34
SP - 1407
EP - 1414
JO - Electrophoresis
JF - Electrophoresis
IS - 9-10
ER -