Regulation of RamA by RamR in Salmonella enterica Serovar Typhimurium: Isolation of a RamR Superrepressor

Vito Ricci; Stephen J W Busby; Laura J V Piddock

doi:10.1128/AAC.01320-12

Regulation of RamA by RamR in Salmonella enterica Serovar Typhimurium: Isolation of a RamR Superrepressor

Vito Ricci, Stephen J W Busby, Laura J V Piddock

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

RamA is a transcription factor involved in regulating multidrug resistance in Salmonella enterica serovar Typhimurium SL1344. Green fluorescent protein (GFP) reporter fusions were exploited to investigate the regulation of RamA expression by RamR. We show that RamR represses the ramA promoter by binding to a palindromic sequence and describe a superrepressor RamR mutant that binds to the ramA promoter sequence more efficiently, thus exhibiting a ramA inactivated phenotype.

Original language	English
Pages (from-to)	6037-40
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	56
Issue number	11
DOIs	https://doi.org/10.1128/AAC.01320-12
Publication status	Published - 2012

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10.1128/AAC.01320-12

Unravelling the Network of Regulation of Multidrug Resistance in Salmonella Enterica
Piddock, L. & Busby, S.
Medical Research Council
1/05/09 → 31/12/14
Project: Research Councils

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title = "Regulation of RamA by RamR in Salmonella enterica Serovar Typhimurium: Isolation of a RamR Superrepressor",

abstract = "RamA is a transcription factor involved in regulating multidrug resistance in Salmonella enterica serovar Typhimurium SL1344. Green fluorescent protein (GFP) reporter fusions were exploited to investigate the regulation of RamA expression by RamR. We show that RamR represses the ramA promoter by binding to a palindromic sequence and describe a superrepressor RamR mutant that binds to the ramA promoter sequence more efficiently, thus exhibiting a ramA inactivated phenotype.",

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AB - RamA is a transcription factor involved in regulating multidrug resistance in Salmonella enterica serovar Typhimurium SL1344. Green fluorescent protein (GFP) reporter fusions were exploited to investigate the regulation of RamA expression by RamR. We show that RamR represses the ramA promoter by binding to a palindromic sequence and describe a superrepressor RamR mutant that binds to the ramA promoter sequence more efficiently, thus exhibiting a ramA inactivated phenotype.

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DO - 10.1128/AAC.01320-12

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JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

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Regulation of RamA by RamR in Salmonella enterica Serovar Typhimurium: Isolation of a RamR Superrepressor

Abstract

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Unravelling the Network of Regulation of Multidrug Resistance in Salmonella Enterica

Cite this