Recent Advances in Metalloproteomics

James P.C. Coverdale, Sirilata Polepalli, Marco A.Z. Arruda, Ana B. Santos da Silva, Alan J. Stewart*, Claudia A. Blindauer*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

51 Downloads (Pure)

Abstract

Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine, and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owing to the complexity of metalloproteomes, but also to the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part, transition metals from the fourth and fifth periods are examined, most of which are xenobiotic and also tend to form more stable and/or inert complexes. A large research area in this respect concerns metallodrug–protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.

Original languageEnglish
Article number104
Number of pages31
JournalBiomolecules
Volume14
Issue number1
DOIs
Publication statusPublished - 13 Jan 2024

Bibliographical note

Funding Information:
This work was funded by the BBSRC (Biological and Biotechnological Sciences Research Council), Grant No BB/V014684/1. We also thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), Grant Nos. 2014/50867-3, 2018/25207-0 and 2020/08543-7, and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnologico), Grant No 303231/2020-3. JPCC thanks the Royal Society of Chemistry for an Enablement grant (E22-1637945680). The APC was funded by MDPI.

Publisher Copyright:
© 2024 by the authors.

Keywords

  • essential metals
  • ligand exchange kinetics
  • metallodrugs
  • metalloproteome
  • xenobiotic metals

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Recent Advances in Metalloproteomics'. Together they form a unique fingerprint.

Cite this