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Abstract
Emerging and re-emerging viruses are a global health concern. Genome sequencing as an approach for monitoring circulating viruses is currently hampered by complex and expensive methods. Untargeted, metagenomic nanopore sequencing can provide genomic information to identify pathogens, prepare for or even prevent outbreaks.
SMART (Switching Mechanism at the 5′ end of RNA Template) is a popular approach for RNA-Seq but most current methods rely on oligo-dT priming to target polyadenylated mRNA molecules. We have developed two random primed SMART-Seq approaches, a sequencing agnostic approach ‘SMART-9N’ and a version compatible rapid adapters available from Oxford Nanopore Technologies ‘Rapid SMART-9N’. The methods were developed using viral isolates, clinical samples, and compared to a gold-standard amplicon-based method. From a Zika virus isolate the SMART-9N approach recovered 10kb of the 10.8kb RNA genome in a single nanopore read. We also obtained full genome coverage at a high depth coverage using the Rapid SMART-9N, which takes only 10 minutes and costs up to 45% less than other methods. We found the limits of detection of these methods to be 6 focus forming units (FFU)/mL with 99.02% and 87.58% genome coverage for SMART-9N and Rapid SMART-9N respectively. Yellow fever virus plasma samples and SARS-CoV-2 nasopharyngeal samples previously confirmed by RT-qPCR with a broad range of Ct-values were selected for validation. Both methods produced greater genome coverage when compared to the multiplex PCR approach and we obtained the longest single read of this study (18.5 kb) with a SARS-CoV-2 clinical sample, 60% of the virus genome using the Rapid SMART-9N method.
This work demonstrates that SMART-9N and Rapid SMART-9N are sensitive, low input, and long-read compatible alternatives for RNA virus detection and genome sequencing and Rapid SMART-9N improves the cost, time, and complexity of laboratory work.
Original language | English |
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Article number | 241 |
Number of pages | 22 |
Journal | Wellcome Open Research |
Volume | 6 |
DOIs | |
Publication status | Published - 24 Apr 2023 |
Bibliographical note
Funding Information:This work was supported by a Medical Research Council-São Paulo Research Foundation (FAPESP) CADDE partnership award (MR/S0195/1 and FAPESP 18/14389-0) (https://caddecentre.org); FAPESP/Newton Funds (FAPESP 2017/08012-9), Bill & Melinda Gates Foundation (INV-034540 and INV-034652), and I.M.C (FAPESP 2018/17176-8). M.S.R. is supported by the Faculdade de Medicina da Universidade de São Paulo (FMUSP). T.M.C. (2019/07544-2), C.A.M.S. (2019/21301-5), F.C.S.S. (2018/25468-9), J.G.J. (2018/17176-8, 2019/12000-1, 18/14389-0) and E.C.S (2018/14389-0) are supported by FAPESP. P.S.A. is supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (88887.596940/2021-00). W.M.S is supported by FAPESP (2017/13981-0, 2019/24251-9), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (408338/2018-0), and the National Institutes of Health (AI120942). D.S.C is supported by the Clarendon Fund and by the Department of Zoology, University of Oxford. M.R.A is supported by CAPES (88887.356527/2019-00). N.R.F. is supported by a Wellcome Trust Royal Society Sir Henry Dale Fellowship (204311). N.J.L. and J.Q. are supported by the Wellcome ARTIC network (206298).
Publisher Copyright:
Copyright: © 2023 Claro IM et al.
Keywords
- diagnostic
- genomic surveillance
- metagenomic
- nanopore sequencing
- RNA virus
- SARS-CoV-2
- YFV
- ZIKV
ASJC Scopus subject areas
- Medicine (miscellaneous)
- General Biochemistry,Genetics and Molecular Biology
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Putting genomic surveillance at the heart of viural epidemic response
Loman, N. (Principal Investigator)
5/09/17 → 4/09/25
Project: Research