Profiling sex-biased gene expression during parthenogenetic reproduction in Daphnia pulex

Brian D Eads, John K Colbourne, Elizabeth Bohuski, Justen Andrews

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)
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Sexual reproduction is a core biological function that is conserved throughout eukaryotic evolution, yet breeding systems are extremely variable. Genome-wide comparative studies can be effectively used to identify genes and regulatory patterns that are constrained to preserve core functions from those that may help to account for the diversity of animal reproductive strategies. We use a custom microarray to investigate gene expression in males and two reproductive stages of females in the crustacean Daphnia pulex. Most Daphnia species reproduce by cyclical parthenogenesis, alternating between sexual and clonal reproduction. Both sex determination and the switch in their mode of reproduction is environmentally induced, making Daphnia an interesting comparative system for the study of sex-biased and reproductive genes.
Original languageEnglish
Article number464
JournalBMC Genomics
Publication statusPublished - 18 Dec 2007


  • Animals
  • Daphnia
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genes, Insect
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Parthenogenesis
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sex Characteristics
  • Transcription, Genetic


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