Profiling sex-biased gene expression during parthenogenetic reproduction in Daphnia pulex

Brian D Eads; John K Colbourne; Elizabeth Bohuski; Justen Andrews

doi:10.1186/1471-2164-8-464

Profiling sex-biased gene expression during parthenogenetic reproduction in Daphnia pulex

Brian D Eads, John K Colbourne, Elizabeth Bohuski, Justen Andrews

Biosciences

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Abstract

Sexual reproduction is a core biological function that is conserved throughout eukaryotic evolution, yet breeding systems are extremely variable. Genome-wide comparative studies can be effectively used to identify genes and regulatory patterns that are constrained to preserve core functions from those that may help to account for the diversity of animal reproductive strategies. We use a custom microarray to investigate gene expression in males and two reproductive stages of females in the crustacean Daphnia pulex. Most Daphnia species reproduce by cyclical parthenogenesis, alternating between sexual and clonal reproduction. Both sex determination and the switch in their mode of reproduction is environmentally induced, making Daphnia an interesting comparative system for the study of sex-biased and reproductive genes.

Original language	English
Article number	464
Journal	BMC Genomics
Volume	8
DOIs	https://doi.org/10.1186/1471-2164-8-464
Publication status	Published - 18 Dec 2007

Keywords

Animals
Daphnia
Female
Gene Expression Profiling
Gene Expression Regulation
Genes, Insect
Male
Oligonucleotide Array Sequence Analysis
Parthenogenesis
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Sex Characteristics
Transcription, Genetic

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10.1186/1471-2164-8-464

Eads_et_al_Profiling_sex-biased_gene_expression_BMC_Genomics_2007
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Checked July 2015
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Cite this

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title = "Profiling sex-biased gene expression during parthenogenetic reproduction in Daphnia pulex",

abstract = "Sexual reproduction is a core biological function that is conserved throughout eukaryotic evolution, yet breeding systems are extremely variable. Genome-wide comparative studies can be effectively used to identify genes and regulatory patterns that are constrained to preserve core functions from those that may help to account for the diversity of animal reproductive strategies. We use a custom microarray to investigate gene expression in males and two reproductive stages of females in the crustacean Daphnia pulex. Most Daphnia species reproduce by cyclical parthenogenesis, alternating between sexual and clonal reproduction. Both sex determination and the switch in their mode of reproduction is environmentally induced, making Daphnia an interesting comparative system for the study of sex-biased and reproductive genes.",

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AU - Eads, Brian D

AU - Colbourne, John K

AU - Bohuski, Elizabeth

AU - Andrews, Justen

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N2 - Sexual reproduction is a core biological function that is conserved throughout eukaryotic evolution, yet breeding systems are extremely variable. Genome-wide comparative studies can be effectively used to identify genes and regulatory patterns that are constrained to preserve core functions from those that may help to account for the diversity of animal reproductive strategies. We use a custom microarray to investigate gene expression in males and two reproductive stages of females in the crustacean Daphnia pulex. Most Daphnia species reproduce by cyclical parthenogenesis, alternating between sexual and clonal reproduction. Both sex determination and the switch in their mode of reproduction is environmentally induced, making Daphnia an interesting comparative system for the study of sex-biased and reproductive genes.

AB - Sexual reproduction is a core biological function that is conserved throughout eukaryotic evolution, yet breeding systems are extremely variable. Genome-wide comparative studies can be effectively used to identify genes and regulatory patterns that are constrained to preserve core functions from those that may help to account for the diversity of animal reproductive strategies. We use a custom microarray to investigate gene expression in males and two reproductive stages of females in the crustacean Daphnia pulex. Most Daphnia species reproduce by cyclical parthenogenesis, alternating between sexual and clonal reproduction. Both sex determination and the switch in their mode of reproduction is environmentally induced, making Daphnia an interesting comparative system for the study of sex-biased and reproductive genes.

KW - Animals

KW - Daphnia

KW - Female

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - Genes, Insect

KW - Male

KW - Oligonucleotide Array Sequence Analysis

KW - Parthenogenesis

KW - Reproducibility of Results

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sex Characteristics

KW - Transcription, Genetic

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DO - 10.1186/1471-2164-8-464

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SN - 1471-2164

VL - 8

JO - BMC Genomics

JF - BMC Genomics

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ER -

Profiling sex-biased gene expression during parthenogenetic reproduction in Daphnia pulex

Abstract

Keywords

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