Prepatterning of Developmental Gene Expression by Modified Histones before Zygotic Genome Activation

LC Lindeman; IS Andersen; AH Reiner; Nan Li; H Aanes; O Ostrup; C Winata; S Mathavan; Ferenc Mueller; P Alestrom; P Collas

doi:10.1016/j.devcel.2011.10.008

Prepatterning of Developmental Gene Expression by Modified Histones before Zygotic Genome Activation

LC Lindeman, IS Andersen, AH Reiner, Nan Li, H Aanes, O Ostrup, C Winata, S Mathavan, Ferenc Mueller, P Alestrom, P Collas

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Abstract

A hallmark of anamniote vertebrate development is a window of embryonic transcription-independent cell divisions before onset of zygotic genome activation (ZGA). Chromatin determinants of ZGA are unexplored; however, marking of developmental genes by modified histones in sperm suggests a predictive role of histone marks for ZGA. In zebrafish, pre-ZGA development for ten cell cycles provides an opportunity to examine whether genomic enrichment in modified histones is present before initiation of transcription. By profiling histone H3 trimethylation on all zebrafish promoters before and after ZGA, we demonstrate here an epigenetic prepatterning of developmental gene expression. This involves pre-ZGA marking of transcriptionally inactive genes involved in homeostatic and developmental regulation by permissive H3K4me3 with or without repressive H3K9me3 or H3K27me3. Our data suggest that histone modifications are instructive for the developmental gene expression program.

Original language	English
Pages (from-to)	993-1004
Number of pages	12
Journal	Developmental Cell
Volume	21
Issue number	6
DOIs	https://doi.org/10.1016/j.devcel.2011.10.008
Publication status	Published - 1 Dec 2011

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title = "Prepatterning of Developmental Gene Expression by Modified Histones before Zygotic Genome Activation",

abstract = "A hallmark of anamniote vertebrate development is a window of embryonic transcription-independent cell divisions before onset of zygotic genome activation (ZGA). Chromatin determinants of ZGA are unexplored; however, marking of developmental genes by modified histones in sperm suggests a predictive role of histone marks for ZGA. In zebrafish, pre-ZGA development for ten cell cycles provides an opportunity to examine whether genomic enrichment in modified histones is present before initiation of transcription. By profiling histone H3 trimethylation on all zebrafish promoters before and after ZGA, we demonstrate here an epigenetic prepatterning of developmental gene expression. This involves pre-ZGA marking of transcriptionally inactive genes involved in homeostatic and developmental regulation by permissive H3K4me3 with or without repressive H3K9me3 or H3K27me3. Our data suggest that histone modifications are instructive for the developmental gene expression program.",

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doi = "10.1016/j.devcel.2011.10.008",

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T1 - Prepatterning of Developmental Gene Expression by Modified Histones before Zygotic Genome Activation

AU - Lindeman, LC

AU - Andersen, IS

AU - Reiner, AH

AU - Li, Nan

AU - Aanes, H

AU - Ostrup, O

AU - Winata, C

AU - Mathavan, S

AU - Mueller, Ferenc

AU - Alestrom, P

AU - Collas, P

PY - 2011/12/1

Y1 - 2011/12/1

N2 - A hallmark of anamniote vertebrate development is a window of embryonic transcription-independent cell divisions before onset of zygotic genome activation (ZGA). Chromatin determinants of ZGA are unexplored; however, marking of developmental genes by modified histones in sperm suggests a predictive role of histone marks for ZGA. In zebrafish, pre-ZGA development for ten cell cycles provides an opportunity to examine whether genomic enrichment in modified histones is present before initiation of transcription. By profiling histone H3 trimethylation on all zebrafish promoters before and after ZGA, we demonstrate here an epigenetic prepatterning of developmental gene expression. This involves pre-ZGA marking of transcriptionally inactive genes involved in homeostatic and developmental regulation by permissive H3K4me3 with or without repressive H3K9me3 or H3K27me3. Our data suggest that histone modifications are instructive for the developmental gene expression program.

AB - A hallmark of anamniote vertebrate development is a window of embryonic transcription-independent cell divisions before onset of zygotic genome activation (ZGA). Chromatin determinants of ZGA are unexplored; however, marking of developmental genes by modified histones in sperm suggests a predictive role of histone marks for ZGA. In zebrafish, pre-ZGA development for ten cell cycles provides an opportunity to examine whether genomic enrichment in modified histones is present before initiation of transcription. By profiling histone H3 trimethylation on all zebrafish promoters before and after ZGA, we demonstrate here an epigenetic prepatterning of developmental gene expression. This involves pre-ZGA marking of transcriptionally inactive genes involved in homeostatic and developmental regulation by permissive H3K4me3 with or without repressive H3K9me3 or H3K27me3. Our data suggest that histone modifications are instructive for the developmental gene expression program.

U2 - 10.1016/j.devcel.2011.10.008

DO - 10.1016/j.devcel.2011.10.008

M3 - Article

C2 - 22137762

VL - 21

SP - 993

EP - 1004

JO - Developmental Cell

JF - Developmental Cell

IS - 6

ER -

Prepatterning of Developmental Gene Expression by Modified Histones before Zygotic Genome Activation

Abstract

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