TY - JOUR
T1 - Preparation and Characterization of a Novel Pyrrole-benzophenone Copolymerized Silica Nanocomposite as a Reagent in a Visual Immunologic-agglutination Test
AU - Goldberg Oppenheimer, Pola
AU - Marks, R S
AU - Regev, Oren
PY - 2008
Y1 - 2008
N2 - Biological sensing is explored through novel stable colloidal dispersions of pyrrole-benzophenone and pyrrole copolymerized silica (PPy-SiO2-PPyBPh) nanocomposites, which allow covalent linking of biological molecules through light mediation. The mechanism of nanocomposite attachment to a model protein is studied by gold labeled cholera toxin B (CTB) to enhance the contrast in electron microscopy imaging. The biological test itself is carried out without gold labeling, i.e., using CTB only. The protein is shown to be covalently bound through the benzophenone groups. When the reactive PPy-SiO2-PPyBPh-CTB nanocomposite is exposed to specific recognition anti-CTB immunoglobulins, a qualitative visual agglutination assay occurs spontaneously, producing as a positive test, PPy-SiO2-PPyBPh-CTB-anti-CTB, in less than 1 h, while the control solution of the PPy-SiO2-PPyBPh-CTB alone remained well-dispersed during the same period. These dispersions were characterized by cryogenic transmission microscopy (cryo-TEM), scanning electron microscopy (SEM), FTIR and X-ray photoelectron spectroscopy (XPS).
AB - Biological sensing is explored through novel stable colloidal dispersions of pyrrole-benzophenone and pyrrole copolymerized silica (PPy-SiO2-PPyBPh) nanocomposites, which allow covalent linking of biological molecules through light mediation. The mechanism of nanocomposite attachment to a model protein is studied by gold labeled cholera toxin B (CTB) to enhance the contrast in electron microscopy imaging. The biological test itself is carried out without gold labeling, i.e., using CTB only. The protein is shown to be covalently bound through the benzophenone groups. When the reactive PPy-SiO2-PPyBPh-CTB nanocomposite is exposed to specific recognition anti-CTB immunoglobulins, a qualitative visual agglutination assay occurs spontaneously, producing as a positive test, PPy-SiO2-PPyBPh-CTB-anti-CTB, in less than 1 h, while the control solution of the PPy-SiO2-PPyBPh-CTB alone remained well-dispersed during the same period. These dispersions were characterized by cryogenic transmission microscopy (cryo-TEM), scanning electron microscopy (SEM), FTIR and X-ray photoelectron spectroscopy (XPS).
U2 - /10.1016/j.talanta.2008.01.060
DO - /10.1016/j.talanta.2008.01.060
M3 - Article
SN - 0039-9140
VL - 75
SP - 1324
EP - 1331
JO - Talanta
JF - Talanta
IS - 5
ER -