Polar body detection for ICSI cell manipulation

Amir M. Hajiyavand, Mozafar Saadat, Ajai Pal Singh Bedi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Polar body detection is an essential activity in the automation of micromanipulation systems in Intracytoplasmic sperm injection (ICSI) application. Orienting the intracellular structure to the desire location is main challenge in the 3D cell manipulation. Polar body is classified as one of these intracellular structures. This paper presents the development and testing of a new oocytes and polar body detection method. The detection and location of oocyte and its polar body were completed using the gradient weighted Hough transform, which was the first documented implementation in this application. A new method for elliptical fitting within images based on image gradients was developed for the sizing of oocytes and polar bodies. The test results showed the robustness of the proposed methodology compared with the established methods to detect oocytes and polar bodies from different sources.

Original languageEnglish
Title of host publication2016 International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2016
EditorsSinan Haliyo, Albert Sill, Stephane Regnier, Sergej Fatikow
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
ISBN (Electronic)9781509015108
DOIs
Publication statusPublished - 6 Sep 2016
Event1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2016 - Paris, France
Duration: 18 Jul 201621 Jul 2016

Publication series

Name2016 International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2016

Conference

Conference1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2016
Country/TerritoryFrance
CityParis
Period18/07/1621/07/16

Keywords

  • ICSI
  • MicroManipulation
  • Oocyte
  • Polar body

ASJC Scopus subject areas

  • Artificial Intelligence
  • Human-Computer Interaction
  • Computer Science Applications

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