Photocurable antimicrobial silk-based hydrogels for corneal repair

Inês A Barroso, Kenny Man, Thomas J Hall, Thomas E Robinson, Sophie E T Louth, Sophie C Cox, Anita K Ghag

Research output: Contribution to journalArticlepeer-review

Abstract

Corneal transplantation is the current gold standard treatment to restore visual acuity to patients with severe corneal diseases and injuries. Due to severe donor tissue shortage, efforts to develop a corneal equivalent have been made but the challenge remains unmet. Another issue of concern in ocular surgery is the difficult instillation and fast drainage of antibiotic ocular eye drops as bacterial infections can jeopardize implant success by delaying or impairing tissue healing. In this study, we developed antimicrobial silk-based hydrogels that have the potential to be photoactivated in situ, fully adapting to the corneal injury shape. Gentamicin-loaded methacrylated-silk (SilkMA) hydrogels were prepared within minutes using low UV intensity (3 mW/cm2 ). SilkMA gels provided a Young's modulus between 21 and 79 kPa together with a light transmittance spectrum and water content (83%-90%) similar to the human cornea. Polymer concentration (15%-25%) was found to offer a tool for tailoring the physical properties of the hydrogels. We confirmed that the methacrylation did not affect the material's in vitro degradation and biocompatibility by observing fibroblast adhesion and proliferation. Importantly, agar diffusion tests showed that the synthesized hydrogels were able to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth for 72 h. These characteristics along with their injectability and viscoelasticity demonstrate the potential of SilkMA hydrogels to be applied in several soft tissue engineering fields. As such, for the first time we demonstrate the potential of photocurable antimicrobial SilkMA hydrogels as a novel biomaterial to facilitate corneal regeneration.

Original languageEnglish
Pages (from-to)1401-1415
Number of pages15
JournalJournal of Biomedical Materials Research Part A
Volume110
Issue number7
Early online date8 Mar 2022
DOIs
Publication statusE-pub ahead of print - 8 Mar 2022

Bibliographical note

© 2022 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.

Keywords

  • Anti-Bacterial Agents
  • Anti-Infective Agents/pharmacology
  • Cornea
  • Fibroins/pharmacology
  • Humans
  • Hydrogels/pharmacology
  • Silk
  • Tissue Engineering

Fingerprint

Dive into the research topics of 'Photocurable antimicrobial silk-based hydrogels for corneal repair'. Together they form a unique fingerprint.

Cite this