Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration

Zubair Ahmed*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Optic nerve crush injury is a useful model for studying the response of central nervous system neurons (CNS) to injury. A particular focus of this model has been to elucidate therapeutic factors in promoting neuroprotection and axon regeneration after injury. Here we describe a step-by-step protocol in accessing the optic nerve and creating a crush injury. This can be used to create a reproducible model to study the response of retinal ganglion cells (RGC), the main projection neurons of the eye, to injury.
Original languageEnglish
Title of host publicationRetinal Ganglion Cells
Subtitle of host publicationMethods and Protocols
EditorsBen Mead
Place of PublicationNew York, NY
PublisherHumana Press
Chapter10
Pages99-105
ISBN (Electronic)9781071634097
ISBN (Print)9781071634080
DOIs
Publication statusPublished - 10 Aug 2023

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume2708
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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