Oncostatin M and Kit-Ligand control hematopoietic stem cell fate during zebrafish embryogenesis

Chris B. Mahony, Corentin Pasche, Julien Y. Bertrand*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)
163 Downloads (Pure)


Understanding the molecular pathways controlling hematopoietic stem cell specification and expansion is a necessary milestone to perform regenerative medicine. Here, we used the zebrafish model to study the role of the ckit signaling pathway in this process. We show the importance of kitb/kitlgb signaling in the specification and expansion of hematopoietic stem cells (HSCs), in the hemogenic endothelium and caudal hematopoietic tissue (CHT), respectively. Moreover, we identified the zebrafish ortholog of Oncostatin M (osm) in the zebrafish genome. We show that the osm/osmr pathway acts upstream of kitb during specification of the hemogenic endothelium, while both pathways act synergistically to expand HSCs in the CHT. Moreover, we found that osm, in addition to its role in promoting HSC proliferation, inhibits HSC commitment to the lymphoid fate. Altogether, our data identified two cytokines, kitlgb and osm, secreted by the vascular niche, that control HSCs during early embryonic development.
Original languageEnglish
Pages (from-to)1920-1934
Number of pages15
JournalStem Cell Reports
Issue number6
Early online date17 May 2018
Publication statusPublished - 5 Jun 2018

Bibliographical note

Funding Information:
We kindly thank Prof. David Traver for the gata2b ISH probe. We also thank Dr. Sylvain Lemeille for his advice on statistical analysis. J.Y.B. was endorsed by a Chair in Life Sciences funded by the Gabriella Giorgi-Cavaglieri Foundation and is also funded by the Swiss National Fund (31003A_166515) and the Fondation Privée des HUG.

Publisher Copyright:
© 2018 The Author(s)


  • hematopoietic stem cells
  • zebrafish
  • oncostatin M
  • kit-ligand
  • hematopoietic niche
  • oncostatin M receptor (osmr)
  • cKIT

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology


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