NTHi-IAV coinfection of macrophages alters infection outcomes and inflammatory responses

Jodie Ackland; Ashley Heinson; David Cleary; Myron Christodoulides; Tom M.A. Wilkinson; Karl Staples

doi:10.1183/23120541.LSC-2022.76

NTHi-IAV coinfection of macrophages alters infection outcomes and inflammatory responses

Jodie Ackland, Ashley Heinson, David Cleary, Myron Christodoulides, Tom M.A. Wilkinson, Karl Staples

Microbes, Infections and Microbiomes

Research output: Contribution to journal › Conference article › peer-review

Abstract

Nontypeable Haemophilus influenzae (NTHi) chronically colonises the airway of individuals with chronic respiratory disease, with persistence suggested to be facilitated by invasion of airway macrophages. Previous data supports an interaction between NTHi colonisation and the risk of viruses exacerbating underlying respiratory diseases. As exacerbations are the main cause of morbidity and mortality of respiratory diseases, the drivers of this increased risk need to be identified.The aim of this work was to investigate whether prior NTHi infection compromises the ability of macrophages to respond to a subsequent viral challenge. A monocyte-derived macrophage (MDM)-NTHi intracellular persistence model was adapted to include coinfection with the influenza A virus (IAV). Compared to pathogen-alone controls, NTHi presence significantly increased by 190% (p

Original language	English
Article number	76
Journal	ERJ Open Research
Volume	8
Issue number	Suppl 8
DOIs	https://doi.org/10.1183/23120541.LSC-2022.76
Publication status	Published - 9 May 2022
Event	ERS Lung Science Conference - Palácio Estoril Hotel, Estoril, Portugal Duration: 10 Mar 2022 → 13 Mar 2022

Bibliographical note

This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.

Keywords

Viruses
Monocyte/Macrophage
Bacteria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1183/23120541.LSC-2022.76

Cite this

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title = "NTHi-IAV coinfection of macrophages alters infection outcomes and inflammatory responses",

abstract = "Nontypeable Haemophilus influenzae (NTHi) chronically colonises the airway of individuals with chronic respiratory disease, with persistence suggested to be facilitated by invasion of airway macrophages. Previous data supports an interaction between NTHi colonisation and the risk of viruses exacerbating underlying respiratory diseases. As exacerbations are the main cause of morbidity and mortality of respiratory diseases, the drivers of this increased risk need to be identified.The aim of this work was to investigate whether prior NTHi infection compromises the ability of macrophages to respond to a subsequent viral challenge. A monocyte-derived macrophage (MDM)-NTHi intracellular persistence model was adapted to include coinfection with the influenza A virus (IAV). Compared to pathogen-alone controls, NTHi presence significantly increased by 190% (p",

keywords = "Viruses, Monocyte/Macrophage, Bacteria",

author = "Jodie Ackland and Ashley Heinson and David Cleary and Myron Christodoulides and Wilkinson, {Tom M.A.} and Karl Staples",

note = "This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.; ERS Lung Science Conference ; Conference date: 10-03-2022 Through 13-03-2022",

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doi = "10.1183/23120541.LSC-2022.76",

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T1 - NTHi-IAV coinfection of macrophages alters infection outcomes and inflammatory responses

AU - Ackland, Jodie

AU - Heinson, Ashley

AU - Cleary, David

AU - Christodoulides, Myron

AU - Wilkinson, Tom M.A.

AU - Staples, Karl

N1 - This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.

PY - 2022/5/9

Y1 - 2022/5/9

N2 - Nontypeable Haemophilus influenzae (NTHi) chronically colonises the airway of individuals with chronic respiratory disease, with persistence suggested to be facilitated by invasion of airway macrophages. Previous data supports an interaction between NTHi colonisation and the risk of viruses exacerbating underlying respiratory diseases. As exacerbations are the main cause of morbidity and mortality of respiratory diseases, the drivers of this increased risk need to be identified.The aim of this work was to investigate whether prior NTHi infection compromises the ability of macrophages to respond to a subsequent viral challenge. A monocyte-derived macrophage (MDM)-NTHi intracellular persistence model was adapted to include coinfection with the influenza A virus (IAV). Compared to pathogen-alone controls, NTHi presence significantly increased by 190% (p

AB - Nontypeable Haemophilus influenzae (NTHi) chronically colonises the airway of individuals with chronic respiratory disease, with persistence suggested to be facilitated by invasion of airway macrophages. Previous data supports an interaction between NTHi colonisation and the risk of viruses exacerbating underlying respiratory diseases. As exacerbations are the main cause of morbidity and mortality of respiratory diseases, the drivers of this increased risk need to be identified.The aim of this work was to investigate whether prior NTHi infection compromises the ability of macrophages to respond to a subsequent viral challenge. A monocyte-derived macrophage (MDM)-NTHi intracellular persistence model was adapted to include coinfection with the influenza A virus (IAV). Compared to pathogen-alone controls, NTHi presence significantly increased by 190% (p

KW - Viruses

KW - Monocyte/Macrophage

KW - Bacteria

U2 - 10.1183/23120541.LSC-2022.76

DO - 10.1183/23120541.LSC-2022.76

M3 - Conference article

SN - 2312-0541

VL - 8

JO - ERJ Open Research

JF - ERJ Open Research

IS - Suppl 8

M1 - 76

T2 - ERS Lung Science Conference

Y2 - 10 March 2022 through 13 March 2022

ER -

NTHi-IAV coinfection of macrophages alters infection outcomes and inflammatory responses

Abstract

Bibliographical note

Keywords

UN SDGs

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Cite this