Neutrophils are important in early control of lung infection by Yersinia pestis

Thomas R Laws; Martin S Davey; Richard W Titball; Roman Lukaszewski

doi:10.1016/j.micinf.2010.01.007

Neutrophils are important in early control of lung infection by Yersinia pestis

Thomas R Laws, Martin S Davey, Richard W Titball, Roman Lukaszewski

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from naïve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect.

Original language	English
Pages (from-to)	331-5
Number of pages	5
Journal	Microbes and Infection
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/j.micinf.2010.01.007
Publication status	Published - Apr 2010

Keywords

Adult
Animals
Cells, Cultured
Humans
Leukocyte Reduction Procedures
Leukocytes, Mononuclear
Lung
Male
Mice
Mice, Inbred BALB C
Microbial Viability
Middle Aged
Neutrophils
Plague
Yersinia pestis
Young Adult

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10.1016/j.micinf.2010.01.007

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title = "Neutrophils are important in early control of lung infection by Yersinia pestis",

abstract = "In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from na{\"i}ve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect.",

keywords = "Adult, Animals, Cells, Cultured, Humans, Leukocyte Reduction Procedures, Leukocytes, Mononuclear, Lung, Male, Mice, Mice, Inbred BALB C, Microbial Viability, Middle Aged, Neutrophils, Plague, Yersinia pestis, Young Adult",

author = "Laws, {Thomas R} and Davey, {Martin S} and Titball, {Richard W} and Roman Lukaszewski",

year = "2010",

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doi = "10.1016/j.micinf.2010.01.007",

language = "English",

volume = "12",

pages = "331--5",

journal = "Microbes and Infection",

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TY - JOUR

T1 - Neutrophils are important in early control of lung infection by Yersinia pestis

AU - Laws, Thomas R

AU - Davey, Martin S

AU - Titball, Richard W

AU - Lukaszewski, Roman

PY - 2010/4

Y1 - 2010/4

N2 - In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from naïve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect.

AB - In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from naïve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect.

KW - Adult

KW - Animals

KW - Cells, Cultured

KW - Humans

KW - Leukocyte Reduction Procedures

KW - Leukocytes, Mononuclear

KW - Lung

KW - Male

KW - Mice

KW - Mice, Inbred BALB C

KW - Microbial Viability

KW - Middle Aged

KW - Neutrophils

KW - Plague

KW - Yersinia pestis

KW - Young Adult

U2 - 10.1016/j.micinf.2010.01.007

DO - 10.1016/j.micinf.2010.01.007

M3 - Article

C2 - 20114086

SN - 1286-4579

VL - 12

SP - 331

EP - 335

JO - Microbes and Infection

JF - Microbes and Infection

IS - 4

ER -

Neutrophils are important in early control of lung infection by Yersinia pestis

Abstract

Keywords

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