Monitoring base excision repair proteins on damaged DNA using human cell extracts

J L Parsons; G L Dianov

doi:10.1042/BST0320962

Monitoring base excision repair proteins on damaged DNA using human cell extracts

J L Parsons, G L Dianov

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

BER (base excision repair) is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). We have developed an assay, using formaldehyde cross-linking during repair in human cell extracts, to observe BER proteins involved in the repair of damaged DNA. This approach allows visualization of repair proteins on damaged DNA during BER in human cell extracts and provides a detailed view of the molecular events leading to repair.

Original language	English
Pages (from-to)	962-3
Number of pages	2
Journal	Biochemical Society Transactions
Volume	32
Issue number	Pt 6
DOIs	https://doi.org/10.1042/BST0320962
Publication status	Published - Dec 2004

Keywords

DNA Damage
DNA Repair/genetics
DNA, Neoplasm/genetics
Formaldehyde
HeLa Cells
Humans

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10.1042/BST0320962

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abstract = "BER (base excision repair) is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). We have developed an assay, using formaldehyde cross-linking during repair in human cell extracts, to observe BER proteins involved in the repair of damaged DNA. This approach allows visualization of repair proteins on damaged DNA during BER in human cell extracts and provides a detailed view of the molecular events leading to repair.",

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AU - Dianov, G L

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AB - BER (base excision repair) is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). We have developed an assay, using formaldehyde cross-linking during repair in human cell extracts, to observe BER proteins involved in the repair of damaged DNA. This approach allows visualization of repair proteins on damaged DNA during BER in human cell extracts and provides a detailed view of the molecular events leading to repair.

KW - DNA Damage

KW - DNA Repair/genetics

KW - DNA, Neoplasm/genetics

KW - Formaldehyde

KW - HeLa Cells

KW - Humans

U2 - 10.1042/BST0320962

DO - 10.1042/BST0320962

M3 - Article

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SP - 962

EP - 963

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - Pt 6

ER -

Monitoring base excision repair proteins on damaged DNA using human cell extracts

Abstract

Keywords

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