Projects per year
Abstract
Microglia provide protection against a range of brain infections including bacteria, viruses and parasites, but how these glial cells respond to fungal brain infections is poorly understood. We investigated the role of microglia in the context of cryptococcal meningitis, the most common cause of fungal meningitis in humans. Using a series of transgenic- and chemical-based microglia depletion methods we found that, contrary to their protective role during other infections, loss of microglia did not affect control of Cryptococcus neoformans brain infection which was replicated with several fungal strains. At early time points post-infection, we found that microglia depletion lowered fungal brain burdens, which was related to intracellular residence of C. neoformans within microglia. Further examination of extracellular and intracellular fungal populations revealed that C. neoformans residing in microglia were protected from copper starvation, whereas extracellular yeast upregulated copper transporter CTR4. However, the degree of copper starvation did not equate to fungal survival or abundance of metals within different intracellular niches. Taken together, these data show how tissue-resident myeloid cells may influence fungal phenotype in the brain but do not provide protection against this infection, and instead may act as an early infection reservoir.
Original language | English |
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Article number | 7202 |
Number of pages | 15 |
Journal | Nature Communications |
Volume | 14 |
Issue number | 1 |
DOIs | |
Publication status | Published - 8 Nov 2023 |
Bibliographical note
Acknowledgments:We would like to thank the technical staff at the Biomedical Services Unit (Birmingham) for their care and help with animal husbandry. In particular, we thank Claire Lyons and Karen Boswell for their time and patience performing infections and caring for our animals. We thank Dr Guillaume Desanti and support staff at the Technology Hub and Flow Cytometry Unit at the University of Birmingham for their support with sorting, flow cytometry and microscopy experiments. This work was funded by the Academy of Medical Sciences (SBF004_1008, awarded to RAD), Medical Research Council (MR/S024611, awarded to RAD), Research Foundation Flanders (PhD studentship awarded to EV, 1SF2222N), the National Natural Science Foundation of China (31870140, awarded to CD) and the Wellcome Trust and the Royal Society (211241/Z/18/Z, awarded to ERB).
Keywords
- Humans
- Meningitis, Cryptococcal/prevention & control
- Microglia
- Copper
- Cryptococcus neoformans
- Neuroglia
- Cryptococcosis
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Fungi on the brain: Interrogating Host-Pathogen Interactions during Fungal Meningitis
Drummond, R. (Principal Investigator)
1/10/19 → 31/01/25
Project: Research Councils
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Sense and respond: Investigating molecular mechanisms regulating Cryptococcus neoformans Titan cell formation in response to bacterial triggers
Ballou, L. (Principal Investigator)
1/10/18 → 20/05/21
Project: Research