Abstract
A major goal in the study of gene regulation is to untangle the transcription-regulatory networks of Escherichia coli and other 'simple' organisms. To do this we must catalogue the binding sites of all transcription factors. ChIP (chromatin immunoprecipitation), combined with DNA microarray analysis, is a powerful tool that permits global patterns of DNA binding to be measured. Here, we discuss the benefits of this approach and the application of this technique to bacterial systems.
Original language | English |
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Pages (from-to) | 754-7 |
Number of pages | 4 |
Journal | Biochemical Society Transactions |
Volume | 36 |
Issue number | Pt 4 |
DOIs | |
Publication status | Published - 1 Aug 2008 |
Keywords
- DNA binding
- Escherichia coli
- microarray
- chromatin immunoprecipitation (ChIP)
- transcription