Projects per year
Abstract
Food fortification programs to reduce iron deficiency anemia require bioavailable forms of iron that do not cause adverse organoleptic effects. Rodent studies show that nano-sized ferric phosphate (NP-FePO4) is as bioavailable as ferrous sulfate, but there is controversy over the mechanism of absorption. We undertook in vitro studies to examine this using a Caco-2 cell model and simulated gastrointestinal (GI) digestion. Supernatant iron concentrations increased inversely with pH, and iron uptake into Caco-2 cells was 2–3 fold higher when NP-FePO4 was digested at pH 1 compared to pH 2. The size and distribution of NP-FePO4 particles during GI digestion was examined using transmission electron microscopy. The d50 of the particle distribution was 413 nm. Using disc centrifugal sedimentation, a high degree of agglomeration in NP-FePO4 following simulated GI digestion was observed, with only 20% of the particles ≤1000 nm. In Caco-2 cells, divalent metal transporter-1 (DMT1) and endocytosis inhibitors demonstrated that NP-FePO4 was mainly absorbed via DMT1. Small particles may be absorbed by clathrin-mediated endocytosis and micropinocytosis. These findings should be considered when assessing the potential of iron nanoparticles for food fortification.
Original language | English |
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Pages (from-to) | 359 |
Journal | Nutrients |
Volume | 9 |
Issue number | 4 |
DOIs | |
Publication status | Published - 4 Apr 2017 |
Keywords
- nano iron
- NP-FePO4
- bioavailability
- Caco-2 cells
- simulated gastrointestinal digestion
- DMT1
- endocytosis
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Dive into the research topics of 'Mechanisms of Iron Uptake from Ferric Phosphate Nanoparticles in Human Intestinal Caco-2 Cells'. Together they form a unique fingerprint.Projects
- 1 Finished
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R8/H10/72 - The Facility for Environmental Nanoparticle and Analysis and Characterisation (FENAC)
Valsami-Jones, E. (Principal Investigator), Hodges, N. (Co-Investigator), Lynch, I. (Co-Investigator), Palmer, R. (Co-Investigator), Chipman, K. (Co-Investigator), Jones, I. (Co-Investigator), Kendall, K. (Co-Investigator), Lead, J. (Co-Investigator) & Preece, J. (Co-Investigator)
Natural Environment Research Council
1/03/09 → 31/03/17
Project: Research