Macrophage inflammatory responses to Non-typeable Haemophilus influenzae (NTHi) are strain-dependent

Jodie Ackland; Karen Osman; C. Mirella Spalluto; David W. Cleary; Myron Christodoulides; Tom Wilkinson; Karl J. Staples

doi:10.1183/13993003.congress-2019.PA5440

Macrophage inflammatory responses to Non-typeable Haemophilus influenzae (NTHi) are strain-dependent

Jodie Ackland, Karen Osman, C. Mirella Spalluto, David W. Cleary, Myron Christodoulides, Tom Wilkinson, Karl J. Staples

Microbiology and Infection

Research output: Contribution to journal › Article › peer-review

Abstract

The airway macrophage is crucial for immune surveillance and pathogen clearance, however, this function is impaired in chronic respiratory diseases such as COPD and asthma. This may lead to colonization of the airways by Nontypeable Haemophilus influenzae (NTHi). NTHi is a heterogeneous organism and this heterogeneity could result in strain-dependent differences in macrophage responses, leading to airway persistence of certain NTHi strains. The aim of this work was to investigate macrophage responses to different clinical strains of NTHi. Strain diversity assessment of eight NTHi clinical isolates was performed using ParSNP. Subsequently, three strains (ST14, 201 and 408) were chosen to infect monocyte-derived macrophages (MDM) isolated from the blood of healthy volunteers to investigate expression of inflammatory pathways by PCR or ELISA. Measurement of NTHi hel gene expression revealed increased presence of ST14 and ST408 compared to ST201 (p

Original language	English
Journal	European Respiratory Journal
Volume	54
Issue number	Suppl 63
DOIs	https://doi.org/10.1183/13993003.congress-2019.PA5440
Publication status	Published - 28 Sept 2019

Bibliographical note

M1 - PA5440

Keywords

Monocyte / Macrophage
Bacteria
Inflammation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1183/13993003.congress-2019.PA5440

Cite this

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title = "Macrophage inflammatory responses to Non-typeable Haemophilus influenzae (NTHi) are strain-dependent",

abstract = "The airway macrophage is crucial for immune surveillance and pathogen clearance, however, this function is impaired in chronic respiratory diseases such as COPD and asthma. This may lead to colonization of the airways by Nontypeable Haemophilus influenzae (NTHi). NTHi is a heterogeneous organism and this heterogeneity could result in strain-dependent differences in macrophage responses, leading to airway persistence of certain NTHi strains. The aim of this work was to investigate macrophage responses to different clinical strains of NTHi. Strain diversity assessment of eight NTHi clinical isolates was performed using ParSNP. Subsequently, three strains (ST14, 201 and 408) were chosen to infect monocyte-derived macrophages (MDM) isolated from the blood of healthy volunteers to investigate expression of inflammatory pathways by PCR or ELISA. Measurement of NTHi hel gene expression revealed increased presence of ST14 and ST408 compared to ST201 (p",

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AU - Ackland, Jodie

AU - Osman, Karen

AU - Spalluto, C. Mirella

AU - Cleary, David W.

AU - Christodoulides, Myron

AU - Wilkinson, Tom

AU - Staples, Karl J.

N1 - M1 - PA5440

PY - 2019/9/28

Y1 - 2019/9/28

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AB - The airway macrophage is crucial for immune surveillance and pathogen clearance, however, this function is impaired in chronic respiratory diseases such as COPD and asthma. This may lead to colonization of the airways by Nontypeable Haemophilus influenzae (NTHi). NTHi is a heterogeneous organism and this heterogeneity could result in strain-dependent differences in macrophage responses, leading to airway persistence of certain NTHi strains. The aim of this work was to investigate macrophage responses to different clinical strains of NTHi. Strain diversity assessment of eight NTHi clinical isolates was performed using ParSNP. Subsequently, three strains (ST14, 201 and 408) were chosen to infect monocyte-derived macrophages (MDM) isolated from the blood of healthy volunteers to investigate expression of inflammatory pathways by PCR or ELISA. Measurement of NTHi hel gene expression revealed increased presence of ST14 and ST408 compared to ST201 (p

KW - Monocyte / Macrophage

KW - Bacteria

KW - Inflammation

U2 - 10.1183/13993003.congress-2019.PA5440

DO - 10.1183/13993003.congress-2019.PA5440

M3 - Article

SN - 0903-1936

VL - 54

JO - European Respiratory Journal

JF - European Respiratory Journal

IS - Suppl 63

ER -

Macrophage inflammatory responses to Non-typeable Haemophilus influenzae (NTHi) are strain-dependent

Abstract

Bibliographical note

Keywords

UN SDGs

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