m7GHub V2.0: an updated database for decoding the N7-methylguanosine (m⁷G) epitranscriptome

With recent progress in mapping N7-methylguanosine (m⁷G) RNA methylation sites, tens of thousands of experimentally validated m⁷G sites have been discovered in various species, shedding light on the significant role of m⁷G modification in regulating numerous biological processes including disease pathogenesis. An integrated resource that enables the sharing, annotation and customized analysis of m⁷G data will greatly facilitate m⁷G studies under various physiological contexts. We previously developed the m7GHub database to host mRNA m⁷G sites identified in the human transcriptome. Here, we present m7GHub v.2.0, an updated resource for a comprehensive collection of m⁷G modifications in various types of RNA across multiple species: an m7GDB database containing 430 898 putative m⁷Gsites identified in 23 species, collected from both widely applied next-generation sequencing (NGS) and the emerging Oxford Nanopore direct RNA sequencing (ONT) techniques; an m7GDiseaseDB hosting 156 206 m⁷G-associated variants (involving addition or removal of an m⁷G site), including 3238 disease-relevant m⁷G-SNPs that may function through epitranscriptome disturbance; and two enhanced analysis modules to perform interactive analyses on the collections of m⁷G sites (m7GFinder) and functional variants (m7GSNPer). We expect that m7Ghub v.2.0 should serve as a valuable centralized resource for studying m⁷G modification. It is freely accessible at: www.rnamd.org/m7GHub2.