Key issues in non-viral gene delivery

CW Pouton, Leonard Seymour

    Research output: Contribution to journalArticle

    312 Citations (Scopus)


    The future of non-viral gene therapy depends on a detailed understanding of the barriers to delivery of polynucleotides. These include physicomechanical barriers, which limit the design of delivery devices, physicochemical barriers that influence self-assembly of colloidal particulate formulations, and biological barriers that compromise delivery of the DNA to its target site. It is important that realistic delivery strategies are adopted for early clinical trials in non-viral gene therapy. In the longer term, it should be possible to improve the efficiency of gene delivery by learning from the attributes which viruses have evolved; attributes that enable translocation of viral components across biological membranes. Assembly of stable, organized virus-like particles will require a higher level of control than current practice. Here, we summarize present knowledge of the biodistribution and cellular interactions of gene delivery systems and consider how improvements in gene delivery will be accomplished in the future.
    Original languageEnglish
    Pages (from-to)187-203
    Number of pages17
    JournalAdvanced Drug Delivery Reviews
    Issue number1-3
    Publication statusPublished - 1 Mar 2001


    Dive into the research topics of 'Key issues in non-viral gene delivery'. Together they form a unique fingerprint.

    Cite this