Abstract
The dinuclear iron(II) supramolecular helicates [Fe2L3]Cl4 (L = C25H20N4) bind to DNA through noncovalent (i.e. , hydrogen-bonding, electrostatic) interactions and exhib- it antimicrobial and anticancer effects. In this study, we show that the helicates condense plasmid DNA with a much higher potency than conventional DNA-condensing agents. Notably, molecules of DNA in the presence of the M enan- tiomer of [Fe2L3]Cl4 do not form intermolecular aggregates typically formed by other condensing agents, such as spermidine or spermine. The helicates inhibit the activity of sev- eral DNA-processing enzymes, such as RNA polymerase, DNA topoisomerase I, deoxyribonuclease I, and site-specific restriction endonucleases. However, the results also indicate that the DNA condensation induced by the helicates does not play a crucial role in these inhibition reactions. The mechanisms for the inhibitory effects of [Fe2L3]Cl4 helicates on DNA-related enzymatic activities have been proposed.
Original language | English |
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Pages (from-to) | 11189-11195 |
Journal | Chemistry: A European Journal |
Volume | 21 |
Issue number | 31 |
Early online date | 24 Jun 2015 |
DOIs | |
Publication status | Published - 27 Jul 2015 |