Abstract
Intracellular infections caused by invasive pathogens continue to prove difficult to combat, due in part to the commonly poor membrane permeability of anti-infective drugs. The aim of this study was to improve the intracellular delivery of one such poorly permeable (but broad-spectrum) anti-infective, gentamicin. Gentamicin was encapsulated into liposomal nanocarriers which were then surface functionalized with InvA497, a bacteria-derived invasion protein. Treatment of HEp-2 cells infected with the enteroinvasive bacteria Yersinia pseudotuberculosis or Salmonella enterica with gentamicin-containing, InvA497-functionalized liposomes resulted in a significantly greater reduction in infection load than treatment with non-functionalized liposomes, indicating that such a bacteriomimetic nanocarrier was not only able to promote successful cellular uptake of gentamicin but was also able to mediate anti-infective drug delivery to both cell cytoplasm and intracellular compartments. The developed InvA497-functionalized liposomal nanocarrier therefore holds great promise as a strategy for improving the therapy of intracellular infections.
Original language | English |
---|---|
Pages (from-to) | 41622-41629 |
Number of pages | 8 |
Journal | RSC Advances |
Volume | 6 |
Issue number | 47 |
DOIs | |
Publication status | Published - 20 Apr 2016 |
Bibliographical note
Publisher Copyright:© 2016 The Royal Society of Chemistry.
ASJC Scopus subject areas
- General Chemistry
- General Chemical Engineering