Invasin-functionalized liposome nanocarriers improve the intracellular delivery of anti-infective drugs

Sara Menina, Hagar Ibrahim Labouta, Rebecca Geyer, Tanja Krause, Sarah Gordon, Petra Dersch, Claus Michael Lehr

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Intracellular infections caused by invasive pathogens continue to prove difficult to combat, due in part to the commonly poor membrane permeability of anti-infective drugs. The aim of this study was to improve the intracellular delivery of one such poorly permeable (but broad-spectrum) anti-infective, gentamicin. Gentamicin was encapsulated into liposomal nanocarriers which were then surface functionalized with InvA497, a bacteria-derived invasion protein. Treatment of HEp-2 cells infected with the enteroinvasive bacteria Yersinia pseudotuberculosis or Salmonella enterica with gentamicin-containing, InvA497-functionalized liposomes resulted in a significantly greater reduction in infection load than treatment with non-functionalized liposomes, indicating that such a bacteriomimetic nanocarrier was not only able to promote successful cellular uptake of gentamicin but was also able to mediate anti-infective drug delivery to both cell cytoplasm and intracellular compartments. The developed InvA497-functionalized liposomal nanocarrier therefore holds great promise as a strategy for improving the therapy of intracellular infections.

Original languageEnglish
Pages (from-to)41622-41629
Number of pages8
JournalRSC Advances
Volume6
Issue number47
DOIs
Publication statusPublished - 20 Apr 2016

Bibliographical note

Publisher Copyright:
© 2016 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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