Invasin-functionalized liposome nanocarriers improve the intracellular delivery of anti-infective drugs

Sara Menina, Hagar Ibrahim Labouta, Rebecca Geyer, Tanja Krause, Sarah Gordon, Petra Dersch, Claus Michael Lehr

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
11 Downloads (Pure)

Abstract

Intracellular infections caused by invasive pathogens continue to prove difficult to combat, due in part to the commonly poor membrane permeability of anti-infective drugs. The aim of this study was to improve the intracellular delivery of one such poorly permeable (but broad-spectrum) anti-infective, gentamicin. Gentamicin was encapsulated into liposomal nanocarriers which were then surface functionalized with InvA497, a bacteria-derived invasion protein. Treatment of HEp-2 cells infected with the enteroinvasive bacteria Yersinia pseudotuberculosis or Salmonella enterica with gentamicin-containing, InvA497-functionalized liposomes resulted in a significantly greater reduction in infection load than treatment with non-functionalized liposomes, indicating that such a bacteriomimetic nanocarrier was not only able to promote successful cellular uptake of gentamicin but was also able to mediate anti-infective drug delivery to both cell cytoplasm and intracellular compartments. The developed InvA497-functionalized liposomal nanocarrier therefore holds great promise as a strategy for improving the therapy of intracellular infections.

Original languageEnglish
Pages (from-to)41622-41629
Number of pages8
JournalRSC Advances
Volume6
Issue number47
DOIs
Publication statusPublished - 20 Apr 2016

Bibliographical note

Publisher Copyright:
© 2016 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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