Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice

Loredana G Bucciarelli; Andreas Pollreisz; Moritz Kebschull; Anjali Ganda; Anastasia Z Kalea; Barry I Hudson; Yu Shan Zou; Evanthia Lalla; Ravichandran Ramasamy; Paolo C Colombo; Ann Marie Schmidt; Shi Fang Yan

doi:10.1177/1479164109338775

Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice

Loredana G Bucciarelli, Andreas Pollreisz, Moritz Kebschull, Anjali Ganda, Anastasia Z Kalea, Barry I Hudson, Yu Shan Zou, Evanthia Lalla, Ravichandran Ramasamy, Paolo C Colombo, Ann Marie Schmidt, Shi Fang Yan

Dentistry

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

OBJECTIVE: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice.

METHOD: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture.

RESULTS: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, Il1b mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-1 mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls.

CONCLUSION: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.

Original language	English
Pages (from-to)	249-61
Number of pages	13
Journal	Diabetes and Vascular Disease Research
Volume	6
Issue number	4
DOIs	https://doi.org/10.1177/1479164109338775
Publication status	Published - Oct 2009

Keywords

Animals
Aorta/immunology
Apoptosis
Caspase 1/genetics
Cell Separation
Cells, Cultured
Diabetes Mellitus, Experimental/genetics
Diabetes Mellitus, Type 1/genetics
Endothelial Cells/immunology
Gene Expression Profiling
Gene Expression Regulation
Inflammation/genetics
Inflammation Mediators/metabolism
Interleukin-1beta/genetics
Male
Mice
Mice, Inbred C57BL
Phenotype
RNA, Messenger/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stress, Physiological/genetics
Time Factors
Vena Cava, Inferior/immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/1479164109338775

Cite this

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title = "Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice",

abstract = "OBJECTIVE: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice.METHOD: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture.RESULTS: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, Il1b mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-1 mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls.CONCLUSION: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.",

keywords = "Animals, Aorta/immunology, Apoptosis, Caspase 1/genetics, Cell Separation, Cells, Cultured, Diabetes Mellitus, Experimental/genetics, Diabetes Mellitus, Type 1/genetics, Endothelial Cells/immunology, Gene Expression Profiling, Gene Expression Regulation, Inflammation/genetics, Inflammation Mediators/metabolism, Interleukin-1beta/genetics, Male, Mice, Mice, Inbred C57BL, Phenotype, RNA, Messenger/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Stress, Physiological/genetics, Time Factors, Vena Cava, Inferior/immunology",

author = "Bucciarelli, {Loredana G} and Andreas Pollreisz and Moritz Kebschull and Anjali Ganda and Kalea, {Anastasia Z} and Hudson, {Barry I} and Zou, {Yu Shan} and Evanthia Lalla and Ravichandran Ramasamy and Colombo, {Paolo C} and Schmidt, {Ann Marie} and Yan, {Shi Fang}",

year = "2009",

month = oct,

doi = "10.1177/1479164109338775",

language = "English",

volume = "6",

pages = "249--61",

journal = "Diabetes and Vascular Disease Research",

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number = "4",

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TY - JOUR

T1 - Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice

AU - Bucciarelli, Loredana G

AU - Pollreisz, Andreas

AU - Kebschull, Moritz

AU - Ganda, Anjali

AU - Kalea, Anastasia Z

AU - Hudson, Barry I

AU - Zou, Yu Shan

AU - Lalla, Evanthia

AU - Ramasamy, Ravichandran

AU - Colombo, Paolo C

AU - Schmidt, Ann Marie

AU - Yan, Shi Fang

PY - 2009/10

Y1 - 2009/10

N2 - OBJECTIVE: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice.METHOD: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture.RESULTS: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, Il1b mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-1 mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls.CONCLUSION: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.

AB - OBJECTIVE: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice.METHOD: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture.RESULTS: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, Il1b mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-1 mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls.CONCLUSION: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.

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KW - Diabetes Mellitus, Type 1/genetics

KW - Endothelial Cells/immunology

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - Inflammation/genetics

KW - Inflammation Mediators/metabolism

KW - Interleukin-1beta/genetics

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Phenotype

KW - RNA, Messenger/metabolism

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Stress, Physiological/genetics

KW - Time Factors

KW - Vena Cava, Inferior/immunology

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DO - 10.1177/1479164109338775

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SP - 249

EP - 261

JO - Diabetes and Vascular Disease Research

JF - Diabetes and Vascular Disease Research

IS - 4

ER -

Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice

Abstract

Keywords

UN SDGs

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