Infectio: A generic framework for computational simulation of virus transmission between cells

Artur Yakimovich, Yauhen Yakimovich, Michael Schmid, Jason Mercer, Ivo F. Sbalzarini, Urs F. Greber*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)
132 Downloads (Pure)

Abstract

Viruses spread between cells, tissues, and organisms by cell-free and cell-cell mechanisms, depending on the cell type, the nature of the virus, or the phase of the infection cycle. The mode of viral transmission has a large impact on disease development, the outcome of antiviral therapies or the efficacy of gene therapy protocols. The transmission mode of viruses can be addressed in tissue culture systems using live-cell imaging. Yet even in relatively simple cell cultures, the mechanisms of viral transmission are difficult to distinguish. Here we present a crossplatform software framework called "Infectio," which is capable of simulating transmission phenotypes in tissue culture of virtually any virus. Infectio can estimate interdependent biological parameters, for example for vaccinia virus infection, and differentiate between cell-cell and cell-free virus spreading. Infectio assists in elucidating virus transmission mechanisms, a feature useful for designing strategies of perturbing or enhancing viral transmission. The complexity of the Infectio software is low compared to that of other software commonly used to quantitate features of cell biological images, which yields stable and relatively error-free output from Infectio. The software is open source (GPLv3 license), and operates on the major platforms (Windows, Mac, and Linux). The complete source code can be downloaded from http://infectio.github.io/index.html.

Original languageEnglish
Article numbere00078-15
JournalmSphere
Volume1
Issue number1
DOIs
Publication statusPublished - 10 Feb 2016

Keywords

  • Advection
  • Cell population
  • Cellular automata
  • Convection
  • Diffusion
  • Fluorescence microscopy
  • Hybrid modeling
  • Infection spread
  • Multiscale modeling
  • Numerical simulation
  • Particle strength exchange
  • Phenotypic properties

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Infectio: A generic framework for computational simulation of virus transmission between cells'. Together they form a unique fingerprint.

Cite this