Induction and transcriptional regulation of the co-inhibitory gene module in T cells

Norio Chihara, Asaf Madi, Takaaki Kondo, Huiyuan Zhang, Nandini Acharya, Meromit Singer, Jackson Nyman, Nemanja D. Marjanovic, Monika S. Kowalczyk, Chao Wang, Sema Kurtulus, Travis Law, Yasaman Etminan, James Nevin, Christopher Buckley, Patrick R. Burkett, Jason D. Buenrostro, Orit Rozenblatt-Rosen, Ana C. Anderson, Aviv RegevVijay K. Kuchroo

Research output: Contribution to journalArticlepeer-review

127 Citations (Scopus)
395 Downloads (Pure)

Abstract

The expression of co-inhibitory receptors, such as CTLA-4 and PD-1, on effector T cells is a key mechanism for ensuring immune homeostasis. Dysregulated expression of co-inhibitory receptors on CD4+ T cells promotes autoimmunity, whereas sustained overexpression on CD8+ T cells promotes T cell dysfunction or exhaustion, leading to impaired ability to clear chronic viral infections and diseases such as cancer. Here, using RNA and protein expression profiling at single-cell resolution in mouse cells, we identify a module of co-inhibitory receptors that includes not only several known co-inhibitory receptors (PD-1, TIM-3, LAG-3 and TIGIT) but also many new surface receptors. We functionally validated two new co-inhibitory receptors, activated protein C receptor (PROCR) and podoplanin (PDPN). The module of co-inhibitory receptors is co-expressed in both CD4+ and CD8+ T cells and is part of a larger co-inhibitory gene program that is shared by non-responsive T cells in several physiological contexts and is driven by the immunoregulatory cytokine IL-27. Computational analysis identified the transcription factors PRDM1 and c-MAF as cooperative regulators of the co-inhibitory module, and this was validated experimentally. This molecular circuit underlies the co-expression of co-inhibitory receptors in T cells and identifies regulators of T cell function with the potential to control autoimmunity and tumour immunity.

Original languageEnglish
Pages (from-to)454–459
Number of pages6
JournalNature
Volume558
Issue number7710
Early online date13 Jun 2018
DOIs
Publication statusPublished - 21 Jun 2018

Fingerprint

Dive into the research topics of 'Induction and transcriptional regulation of the co-inhibitory gene module in T cells'. Together they form a unique fingerprint.

Cite this