Human MAIT and CD8 alpha alpha cells develop from a pool of type-17 precommitted CD8(+) T cells

LJ Walker; YH Kang; MO Smith; H Tharmalingham; N Ramamurthy; VM Fleming; N Sahgal; A Leslie; Ye Htun Oo; A Geremia; TJ Scriba; WA Hanekom; GM Lauer; O Lantz; David Adams; F Powrie; E Barnes; P Klenerman

doi:10.1182/blood-2011-05-353789

Human MAIT and CD8 alpha alpha cells develop from a pool of type-17 precommitted CD8(+) T cells

LJ Walker, YH Kang, MO Smith, H Tharmalingham, N Ramamurthy, VM Fleming, N Sahgal, A Leslie, Ye Htun Oo, A Geremia, TJ Scriba, WA Hanekom, GM Lauer, O Lantz, David Adams, F Powrie, E Barnes, P Klenerman

Immunology and Immunotherapy

Research output: Contribution to journal › Article

182 Citations (Scopus)

Abstract

Human mucosal associated invariant T (MAIT) CD8(+) and Tc17 cells are important tissue-homing cell populations, characterized by high expression of CD161 ((++)) and type-17 differentiation, but their origins and relationships remain poorly defined. By transcriptional and functional analyses, we demonstrate that a pool of polyclonal, precommitted type-17 CD161(++)CD8 alpha beta(+) T cells exist in cord blood, from which a prominent MAIT cell (TCR V alpha 7.2(+)) population emerges postnatally. During this expansion, CD8 alpha alpha T cells appear exclusively within a CD161(++)CD8(+)/MAIT subset, sharing cytokine production, chemokine-receptor expression, TCR-usage, and transcriptional profiles with their CD161(++)CD8 alpha beta(+) counterparts. Our data demonstrate the origin and differentiation pathway of MAIT-cells from a naive type-17 precommitted CD161(++)CD8(+) T-cell pool and the distinct phenotype and function of CD8 alpha alpha cells in man. (Blood. 2012;119(2):422-433)

Original language	English
Pages (from-to)	422-433
Number of pages	12
Journal	Blood
Volume	119
Issue number	2
DOIs	https://doi.org/10.1182/blood-2011-05-353789
Publication status	Published - 1 Jan 2012

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10.1182/blood-2011-05-353789

Intrahepatic Signals Involve in the Recruitment and Differentiation of IL-17 Secreting T Cells and Regulatory T Cells in Chronic Hepatitis - Fellowship
Oo, Y. (Principal Investigator)
Medical Research Council
4/01/12 → 5/01/18
Project: Research Councils
The Molecular Basis of Regulatory T Cell Recruitment to the Inflamed Liver
Adams, D. (Principal Investigator)
Medical Research Council
2/10/06 → 30/09/09
Project: Research Councils

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Walker, LJ., Kang, YH., Smith, MO., Tharmalingham, H., Ramamurthy, N., Fleming, VM., Sahgal, N., Leslie, A., Oo, Y. H., Geremia, A., Scriba, TJ., Hanekom, WA., Lauer, GM., Lantz, O., Adams, D., Powrie, F., Barnes, E., & Klenerman, P. (2012). Human MAIT and CD8 alpha alpha cells develop from a pool of type-17 precommitted CD8(+) T cells. Blood, 119(2), 422-433. https://doi.org/10.1182/blood-2011-05-353789

@article{e0ba07e6abe848faa39ac6817f240e70,

title = "Human MAIT and CD8 alpha alpha cells develop from a pool of type-17 precommitted CD8(+) T cells",

abstract = "Human mucosal associated invariant T (MAIT) CD8(+) and Tc17 cells are important tissue-homing cell populations, characterized by high expression of CD161 ((++)) and type-17 differentiation, but their origins and relationships remain poorly defined. By transcriptional and functional analyses, we demonstrate that a pool of polyclonal, precommitted type-17 CD161(++)CD8 alpha beta(+) T cells exist in cord blood, from which a prominent MAIT cell (TCR V alpha 7.2(+)) population emerges postnatally. During this expansion, CD8 alpha alpha T cells appear exclusively within a CD161(++)CD8(+)/MAIT subset, sharing cytokine production, chemokine-receptor expression, TCR-usage, and transcriptional profiles with their CD161(++)CD8 alpha beta(+) counterparts. Our data demonstrate the origin and differentiation pathway of MAIT-cells from a naive type-17 precommitted CD161(++)CD8(+) T-cell pool and the distinct phenotype and function of CD8 alpha alpha cells in man. (Blood. 2012;119(2):422-433)",

author = "LJ Walker and YH Kang and MO Smith and H Tharmalingham and N Ramamurthy and VM Fleming and N Sahgal and A Leslie and Oo, {Ye Htun} and A Geremia and TJ Scriba and WA Hanekom and GM Lauer and O Lantz and David Adams and F Powrie and E Barnes and P Klenerman",

year = "2012",

month = jan,

day = "1",

doi = "10.1182/blood-2011-05-353789",

language = "English",

volume = "119",

pages = "422--433",

journal = "Blood",

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publisher = "American Society of Hematology",

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Walker, LJ, Kang, YH, Smith, MO, Tharmalingham, H, Ramamurthy, N, Fleming, VM, Sahgal, N, Leslie, A, Oo, YH, Geremia, A, Scriba, TJ, Hanekom, WA, Lauer, GM, Lantz, O, Adams, D, Powrie, F, Barnes, E & Klenerman, P 2012, 'Human MAIT and CD8 alpha alpha cells develop from a pool of type-17 precommitted CD8(+) T cells', Blood, vol. 119, no. 2, pp. 422-433. https://doi.org/10.1182/blood-2011-05-353789

TY - JOUR

T1 - Human MAIT and CD8 alpha alpha cells develop from a pool of type-17 precommitted CD8(+) T cells

AU - Walker, LJ

AU - Kang, YH

AU - Smith, MO

AU - Tharmalingham, H

AU - Ramamurthy, N

AU - Fleming, VM

AU - Sahgal, N

AU - Leslie, A

AU - Oo, Ye Htun

AU - Geremia, A

AU - Scriba, TJ

AU - Hanekom, WA

AU - Lauer, GM

AU - Lantz, O

AU - Adams, David

AU - Powrie, F

AU - Barnes, E

AU - Klenerman, P

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Human mucosal associated invariant T (MAIT) CD8(+) and Tc17 cells are important tissue-homing cell populations, characterized by high expression of CD161 ((++)) and type-17 differentiation, but their origins and relationships remain poorly defined. By transcriptional and functional analyses, we demonstrate that a pool of polyclonal, precommitted type-17 CD161(++)CD8 alpha beta(+) T cells exist in cord blood, from which a prominent MAIT cell (TCR V alpha 7.2(+)) population emerges postnatally. During this expansion, CD8 alpha alpha T cells appear exclusively within a CD161(++)CD8(+)/MAIT subset, sharing cytokine production, chemokine-receptor expression, TCR-usage, and transcriptional profiles with their CD161(++)CD8 alpha beta(+) counterparts. Our data demonstrate the origin and differentiation pathway of MAIT-cells from a naive type-17 precommitted CD161(++)CD8(+) T-cell pool and the distinct phenotype and function of CD8 alpha alpha cells in man. (Blood. 2012;119(2):422-433)

AB - Human mucosal associated invariant T (MAIT) CD8(+) and Tc17 cells are important tissue-homing cell populations, characterized by high expression of CD161 ((++)) and type-17 differentiation, but their origins and relationships remain poorly defined. By transcriptional and functional analyses, we demonstrate that a pool of polyclonal, precommitted type-17 CD161(++)CD8 alpha beta(+) T cells exist in cord blood, from which a prominent MAIT cell (TCR V alpha 7.2(+)) population emerges postnatally. During this expansion, CD8 alpha alpha T cells appear exclusively within a CD161(++)CD8(+)/MAIT subset, sharing cytokine production, chemokine-receptor expression, TCR-usage, and transcriptional profiles with their CD161(++)CD8 alpha beta(+) counterparts. Our data demonstrate the origin and differentiation pathway of MAIT-cells from a naive type-17 precommitted CD161(++)CD8(+) T-cell pool and the distinct phenotype and function of CD8 alpha alpha cells in man. (Blood. 2012;119(2):422-433)

U2 - 10.1182/blood-2011-05-353789

DO - 10.1182/blood-2011-05-353789

M3 - Article

C2 - 22086415

SN - 1528-0020

VL - 119

SP - 422

EP - 433

JO - Blood

JF - Blood

IS - 2

ER -

Human MAIT and CD8 alpha alpha cells develop from a pool of type-17 precommitted CD8(+) T cells

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Projects

Intrahepatic Signals Involve in the Recruitment and Differentiation of IL-17 Secreting T Cells and Regulatory T Cells in Chronic Hepatitis - Fellowship

The Molecular Basis of Regulatory T Cell Recruitment to the Inflamed Liver

Cite this