High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity

Nicholas J Loman; Chrystala Constantinidou; Jacqueline Z M Chan; Mihail Halachev; Martin Sergeant; Charles W Penn; Esther R Robinson; Mark J Pallen

doi:10.1038/nrmicro2850

High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity

Nicholas J Loman, Chrystala Constantinidou, Jacqueline Z M Chan, Mihail Halachev, Martin Sergeant, Charles W Penn, Esther R Robinson, Mark J Pallen

Research output: Contribution to journal › Article › peer-review

290 Citations (Scopus)

Abstract

Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.

Original language	English
Pages (from-to)	599-606
Number of pages	8
Journal	Nature Reviews Microbiology
Volume	10
Issue number	9
DOIs	https://doi.org/10.1038/nrmicro2850
Publication status	Published - 2012

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10.1038/nrmicro2850

Development of robust analytical pipelines for the analysis of microbial community data from clinical samples
Loman, N. (Principal Investigator)
Medical Research Council
1/09/12 → 31/08/15
Project: Research Councils
Acinetobacter baumannii: genomic profiling of an emerging hospital pathogen
Pallen, M. (Principal Investigator) & Constantinidou, M. (Co-Investigator)
Medical Research Council
1/09/10 → 31/08/13
Project: Research Councils

Cite this

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abstract = "Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.",

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AU - Sergeant, Martin

AU - Penn, Charles W

AU - Robinson, Esther R

AU - Pallen, Mark J

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High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity

Abstract

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Projects

Development of robust analytical pipelines for the analysis of microbial community data from clinical samples

Acinetobacter baumannii: genomic profiling of an emerging hospital pathogen

Cite this