Hemoglobin Variant Analysis via Direct Surface Sampling of Dried Blood Spots Coupled with High-Resolution Mass Spectrometry

Rebecca Edwards; Andrew Creese; M Baumert; P Griffiths; Josephine Bunch; Helen Cooper

doi:10.1021/ac1030804

Hemoglobin Variant Analysis via Direct Surface Sampling of Dried Blood Spots Coupled with High-Resolution Mass Spectrometry

Rebecca Edwards, Andrew Creese, M Baumert, P Griffiths, Josephine Bunch, Helen Cooper

Research output: Contribution to journal › Article

84 Citations (Scopus)

Abstract

Hemoglobinopathies are the most common inherited disorders. Newborn blood screening for clinically significant hemoglobin variants, including sickle (HbS), HbC, and HbD, has been adopted in many countries as it is widely acknowledged that early detection improves the outcome. We present a method for determination of Hb variants by direct surface sampling of dried blood spots by use of an Advion Triversa Nanomate automated electrospray system coupled to a high-resolution mass spectrometer. The method involves no sample preparation. It is possible to unambiguously identify homozygous and heterozygous HbS, HbC, and HbD variants in

Original language	English
Pages (from-to)	2265-2270
Number of pages	6
Journal	Analytical Chemistry
Volume	83
Issue number	6
DOIs	https://doi.org/10.1021/ac1030804
Publication status	Published - 15 Mar 2011

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abstract = "Hemoglobinopathies are the most common inherited disorders. Newborn blood screening for clinically significant hemoglobin variants, including sickle (HbS), HbC, and HbD, has been adopted in many countries as it is widely acknowledged that early detection improves the outcome. We present a method for determination of Hb variants by direct surface sampling of dried blood spots by use of an Advion Triversa Nanomate automated electrospray system coupled to a high-resolution mass spectrometer. The method involves no sample preparation. It is possible to unambiguously identify homozygous and heterozygous HbS, HbC, and HbD variants in ",

author = "Rebecca Edwards and Andrew Creese and M Baumert and P Griffiths and Josephine Bunch and Helen Cooper",

year = "2011",

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AU - Edwards, Rebecca

AU - Creese, Andrew

AU - Baumert, M

AU - Griffiths, P

AU - Bunch, Josephine

AU - Cooper, Helen

PY - 2011/3/15

Y1 - 2011/3/15

N2 - Hemoglobinopathies are the most common inherited disorders. Newborn blood screening for clinically significant hemoglobin variants, including sickle (HbS), HbC, and HbD, has been adopted in many countries as it is widely acknowledged that early detection improves the outcome. We present a method for determination of Hb variants by direct surface sampling of dried blood spots by use of an Advion Triversa Nanomate automated electrospray system coupled to a high-resolution mass spectrometer. The method involves no sample preparation. It is possible to unambiguously identify homozygous and heterozygous HbS, HbC, and HbD variants in

AB - Hemoglobinopathies are the most common inherited disorders. Newborn blood screening for clinically significant hemoglobin variants, including sickle (HbS), HbC, and HbD, has been adopted in many countries as it is widely acknowledged that early detection improves the outcome. We present a method for determination of Hb variants by direct surface sampling of dried blood spots by use of an Advion Triversa Nanomate automated electrospray system coupled to a high-resolution mass spectrometer. The method involves no sample preparation. It is possible to unambiguously identify homozygous and heterozygous HbS, HbC, and HbD variants in

U2 - 10.1021/ac1030804

DO - 10.1021/ac1030804

M3 - Article

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SN - 1520-6882

VL - 83

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EP - 2270

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 6

ER -

Hemoglobin Variant Analysis via Direct Surface Sampling of Dried Blood Spots Coupled with High-Resolution Mass Spectrometry

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