Glia dictate pioneer axon trajectories in the Drosophila embryonic CNS

A Hidalgo, G E Booth

Research output: Contribution to journalArticlepeer-review

101 Citations (Scopus)


Whereas considerable progress has been made in understanding the molecular mechanisms of axon guidance across the midline, it is still unclear how the axonal trajectories of longitudinal pioneer neurons, which never cross the midline, are established. Here we show that longitudinal glia of the embryonic Drosophila CNS direct formation of pioneer axon pathways. By ablation and analysis of glial cells missing mutants, we demonstrate that glia are required for two kinds of processes. Firstly, glia are required for growth cone guidance, although this requirement is not absolute. We show that the route of extending growth cones is rich in neuronal cell bodies and glia, and also in long processes from both these cell types. Interactions between neurons, glia and their long processes orient extending growth cones. Secondly, glia direct the fasciculation and defasciculation of axons, which pattern the pioneer pathways. Together these events are essential for the selective fasciculation of follower axons along the longitudinal pathways.

Original languageEnglish
Pages (from-to)393-402
Number of pages10
JournalDevelopment (Cambridge)
Issue number2
Publication statusPublished - Jan 2000


  • Animals
  • Axons
  • Cell Movement
  • Central Nervous System
  • DNA-Binding Proteins
  • Drosophila
  • Drosophila Proteins
  • Growth Cones
  • Immunohistochemistry
  • Mutation
  • Neuroglia
  • Neuropeptides
  • Trans-Activators
  • Transcription Factors
  • Journal Article
  • Research Support, Non-U.S. Gov't


Dive into the research topics of 'Glia dictate pioneer axon trajectories in the Drosophila embryonic CNS'. Together they form a unique fingerprint.

Cite this