Abstract
Urosepsis is a bacteraemia infection caused by an organism previously causing an infection in the urinary tract of a patient, a diagnosis which has been classically confirmed by culture of the same species of bacteria from both blood and urine samples. Given the new insights afforded by sequencing technologies into the complicated population structures of infectious agents affecting humans, we sought to investigate urosepsis by comparing the genome sequences of blood and urine isolates of Escherichia coli from five patients with urosepsis. The results confirm the classical urosepsis hypothesis in four of the five cases, but also show the complex nature of extra-intestinal E. coli infection in the fifth case, where three distinct strains caused two distinct infections. Additionally, we show there is little to no variation in the bacterial genome as it progressed from urine to blood, and also present a minimal set of virulence genes required for bacteraemia in E. coli based on gene association. These suggest that most E. coli have the genetic propensity to cause bacteraemia. Clinical Microbiology and Infection
| Original language | English |
|---|---|
| Pages (from-to) | E328-E334 |
| Journal | Clinical Microbiology and Infection |
| Volume | 19 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 2013 |
Bibliographical note
Funding Information:This work was funded by an EMDA healthcare iNET funding award given jointly to MD and AM. Genome sequences of all strains except P5U2 (performed by KP) were performed by Source Bioscience. We would also like to acknowledge the work of Andy Frey and Jade Gouriet in technical assistance. The study information and raw sequence files have been deposited in the Sequence Read Archive at the European Nucleotide Archive (ENA) under accession number ERP001898.
Keywords
- E. coli
- Escherichia coli
- Extra-intestinal pathogenic
- Genomics
- Urosepsis
- Virulence
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
