TY - JOUR
T1 - Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors.
AU - Halsall, John
AU - Gupta, Vibhore
AU - O'Neill, Laura
AU - Turner, Bryan
AU - Nightingale, Karl
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Histone deacetylase inhibitors (HDACi) are increasingly used as therapeutic agents, but the mechanisms by which they alter cell behaviour remain unclear. Here we use microarray expression analysis to show that only a small proportion of genes (∼9%) have altered transcript levels after treating HL60 cells with different HDACi (valproic acid, Trichostatin A, suberoylanilide hydroxamic acid). Different gene populations respond to each inhibitor, with as many genes down- as up-regulated. Surprisingly, HDACi rarely induced increased histone acetylation at gene promoters, with most genes examined showing minimal change, irrespective of whether genes were up- or down-regulated. Many genes seem to be sheltered from the global histone hyperacetyation induced by HDACi.
AB - Histone deacetylase inhibitors (HDACi) are increasingly used as therapeutic agents, but the mechanisms by which they alter cell behaviour remain unclear. Here we use microarray expression analysis to show that only a small proportion of genes (∼9%) have altered transcript levels after treating HL60 cells with different HDACi (valproic acid, Trichostatin A, suberoylanilide hydroxamic acid). Different gene populations respond to each inhibitor, with as many genes down- as up-regulated. Surprisingly, HDACi rarely induced increased histone acetylation at gene promoters, with most genes examined showing minimal change, irrespective of whether genes were up- or down-regulated. Many genes seem to be sheltered from the global histone hyperacetyation induced by HDACi.
U2 - 10.1371/journal.pone.0033453
DO - 10.1371/journal.pone.0033453
M3 - Article
C2 - 22479401
SN - 1932-6203
VL - 7
SP - e33453
JO - PLoS ONE
JF - PLoS ONE
IS - 3
ER -