Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos

Gyulin Hwang; Ferenc Müller; M Aziz Rahman; Darren W Williams; Paul J Murdock; K John Pasi; Geoffrey Goldspink; Hamid Farahmand; Norman Maclean

doi:10.1007/s10126-004-3121-2

Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos

Gyulin Hwang, Ferenc Müller, M Aziz Rahman, Darren W Williams, Paul J Murdock, K John Pasi, Geoffrey Goldspink, Hamid Farahmand, Norman Maclean

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.

Original language	English
Pages (from-to)	485-92
Number of pages	8
Journal	Marine Biotechnology
Volume	6
Issue number	5
DOIs	https://doi.org/10.1007/s10126-004-3121-2
Publication status	Published - 7 May 2004

Keywords

Animals
Bioreactors
Blood Coagulation
Cytomegalovirus
DNA, Complementary
Enzyme-Linked Immunosorbent Assay
Factor VII
Fishes
Genetic Vectors
Humans
Microinjections
Photometry
Promoter Regions, Genetic
Transgenes
Zygote

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10.1007/s10126-004-3121-2

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title = "Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos",

abstract = "A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.",

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doi = "10.1007/s10126-004-3121-2",

language = "English",

volume = "6",

pages = "485--92",

journal = "Marine Biotechnology",

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TY - JOUR

T1 - Fish as bioreactors

T2 - transgene expression of human coagulation factor VII in fish embryos

AU - Hwang, Gyulin

AU - Müller, Ferenc

AU - Rahman, M Aziz

AU - Williams, Darren W

AU - Murdock, Paul J

AU - Pasi, K John

AU - Goldspink, Geoffrey

AU - Farahmand, Hamid

AU - Maclean, Norman

PY - 2004/5/7

Y1 - 2004/5/7

N2 - A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.

AB - A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.

KW - Animals

KW - Bioreactors

KW - Blood Coagulation

KW - Cytomegalovirus

KW - DNA, Complementary

KW - Enzyme-Linked Immunosorbent Assay

KW - Factor VII

KW - Fishes

KW - Genetic Vectors

KW - Humans

KW - Microinjections

KW - Photometry

KW - Promoter Regions, Genetic

KW - Transgenes

KW - Zygote

U2 - 10.1007/s10126-004-3121-2

DO - 10.1007/s10126-004-3121-2

M3 - Article

C2 - 15129328

SN - 1436-2228

VL - 6

SP - 485

EP - 492

JO - Marine Biotechnology

JF - Marine Biotechnology

IS - 5

ER -

Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos

Abstract

Keywords

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