Embryonic cell ablation in Drosophila using lasers

Sean T Sweeney; Alicia Hidalgo; J Steven de Belle; Haig Keshishian

doi:10.1101/pdb.prot068395

Embryonic cell ablation in Drosophila using lasers

Sean T Sweeney, Alicia Hidalgo, J Steven de Belle, Haig Keshishian

Biosciences

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

Cell ablation is a powerful tool in the study of eukaryotic developmental biology. The selective removal of cells by ablation may provide much information about their origin, fate, or function in the developing organism. Laser-based techniques have an advantage over genetic or chemical ablation methods in that the operations can be performed in essentially any cell pattern and at any time in development. This protocol describes the methods needed to target and ablate specific cells of interest in Drosophila embryos with lasers.

Original language	English
Pages (from-to)	691-3
Number of pages	3
Journal	Cold Spring Harbor Protocols
Volume	2012
Issue number	6
DOIs	https://doi.org/10.1101/pdb.prot068395
Publication status	Published - 1 Jun 2012

Keywords

Ablation Techniques
Animals
Drosophila
Entomology
Lasers
Journal Article
Research Support, Non-U.S. Gov't

Access to Document

10.1101/pdb.prot068395

Cite this

@article{c55982267d2e4ed59eebb90a583457f4,

title = "Embryonic cell ablation in Drosophila using lasers",

abstract = "Cell ablation is a powerful tool in the study of eukaryotic developmental biology. The selective removal of cells by ablation may provide much information about their origin, fate, or function in the developing organism. Laser-based techniques have an advantage over genetic or chemical ablation methods in that the operations can be performed in essentially any cell pattern and at any time in development. This protocol describes the methods needed to target and ablate specific cells of interest in Drosophila embryos with lasers.",

keywords = "Ablation Techniques, Animals, Drosophila, Entomology, Lasers, Journal Article, Research Support, Non-U.S. Gov't",

author = "Sweeney, {Sean T} and Alicia Hidalgo and {de Belle}, {J Steven} and Haig Keshishian",

year = "2012",

month = jun,

day = "1",

doi = "10.1101/pdb.prot068395",

language = "English",

volume = "2012",

pages = "691--3",

journal = "Cold Spring Harbor Protocols",

issn = "1940-3402",

publisher = "Cold Spring Harbor Laboratory Press",

number = "6",

}

TY - JOUR

T1 - Embryonic cell ablation in Drosophila using lasers

AU - Sweeney, Sean T

AU - Hidalgo, Alicia

AU - de Belle, J Steven

AU - Keshishian, Haig

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Cell ablation is a powerful tool in the study of eukaryotic developmental biology. The selective removal of cells by ablation may provide much information about their origin, fate, or function in the developing organism. Laser-based techniques have an advantage over genetic or chemical ablation methods in that the operations can be performed in essentially any cell pattern and at any time in development. This protocol describes the methods needed to target and ablate specific cells of interest in Drosophila embryos with lasers.

AB - Cell ablation is a powerful tool in the study of eukaryotic developmental biology. The selective removal of cells by ablation may provide much information about their origin, fate, or function in the developing organism. Laser-based techniques have an advantage over genetic or chemical ablation methods in that the operations can be performed in essentially any cell pattern and at any time in development. This protocol describes the methods needed to target and ablate specific cells of interest in Drosophila embryos with lasers.

KW - Ablation Techniques

KW - Animals

KW - Drosophila

KW - Entomology

KW - Lasers

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1101/pdb.prot068395

DO - 10.1101/pdb.prot068395

M3 - Article

C2 - 22661443

SN - 1940-3402

VL - 2012

SP - 691

EP - 693

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 6

ER -

Embryonic cell ablation in Drosophila using lasers

Abstract

Keywords

Access to Document

Fingerprint

Cite this