Effects of filtration and pH perturbation on organic matter fluorescence

Andrew Baker, Sarah Elliott, Jamie Lead

Research output: Contribution to journalArticle

74 Citations (Scopus)


Fluorescence of organic matter from six contrasting freshwaters was analysed after filtration (1.2 microm and 0.2 microm filter sizes) and pH perturbation (+/-2 pH units from ambient conditions). Two fluorophores were compared in detail: tryptophan-like fluorescence, whose filtration and pH characteristics are relatively poorly understood, and humic-like fluorescence, which is better characterised. Although there was some variability in both fluorophores, the tryptophan-like fluorescence showed the most significant decrease in fluorescence intensity between raw and 1.2 microm filter samples, and a much smaller decrease between 1.2 and 0.2 microm, demonstrating a significant source associated with particulate material as well as a significant
Original languageEnglish
Pages (from-to)2035-2043
Number of pages9
Publication statusPublished - 1 May 2007


  • humic substances
  • fluorescence
  • filtration
  • organic matter
  • pH


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