TY - JOUR
T1 - Dual RNASeq unveils NTHi-macrophage transcriptomic changes during intracellular persistence
AU - Ackland, Jodie
AU - Heinson, Ashley
AU - Cleary, David
AU - Christodoulides, Myron
AU - Wilkinson, Tom
AU - Staples, Karl
PY - 2020/10/28
Y1 - 2020/10/28
N2 - Nontypeable Haemophilus influenzae (NTHi) is a respiratory tract pathogen associated with severe, neutrophilic asthma. Although macrophages are responsible for orchestrating the immune response and pathogen clearance in the lung, NTHi is able to persist within macrophages. The mechanism of NTHi intracellular persistence is not understood, therefore the aim of this work was to use dual RNASeq to investigate the host-pathogen interactions that allow this persistence. Monocyte-derived macrophages (MDM) were used to model NTHi-macrophage infection. RNA was isolated after 6h and 24h of infection and sequenced using the Illumina NovaSeq 6000 platform. Differential gene expression analysis found expression of 863 MDM genes (FDR p
AB - Nontypeable Haemophilus influenzae (NTHi) is a respiratory tract pathogen associated with severe, neutrophilic asthma. Although macrophages are responsible for orchestrating the immune response and pathogen clearance in the lung, NTHi is able to persist within macrophages. The mechanism of NTHi intracellular persistence is not understood, therefore the aim of this work was to use dual RNASeq to investigate the host-pathogen interactions that allow this persistence. Monocyte-derived macrophages (MDM) were used to model NTHi-macrophage infection. RNA was isolated after 6h and 24h of infection and sequenced using the Illumina NovaSeq 6000 platform. Differential gene expression analysis found expression of 863 MDM genes (FDR p
U2 - 10.1183/13993003.congress-2020.2323
DO - 10.1183/13993003.congress-2020.2323
M3 - Article
SN - 0903-1936
VL - 56
JO - European Respiratory Journal
JF - European Respiratory Journal
IS - Suppl. 64
ER -