DNA barcoding of the genus Verbascum (Scrophulariaceae) in the Arabian Peninsula

Ali Mohammed Alzahrani; Joana Magos Brehm; Shahina A. Ghazanfar; Nigel Maxted

doi:10.1002/tax.13156

DNA barcoding of the genus Verbascum (Scrophulariaceae) in the Arabian Peninsula

Ali Mohammed Alzahrani^*, Joana Magos Brehm, Shahina A. Ghazanfar, Nigel Maxted

^*Corresponding author for this work

Biosciences

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Abstract

Verbascum and Rhabdotosperma are members of the family Scrophulariaceae. The first genus comprises approximately 360 species from almost all parts of the world, while the second contains a total of 8 species from tropical Africa and the Arabian Peninsula. Since 1977, the relationships between Verbascum and Rhabdotosperma continue to be contested. The present study aims to present the phylogenetic relationships and status among Verbascum species in the Arabian Peninsula. For phylogenetic analyses, maximum parsimony and Bayesian inference were performed. In total, 236 DNA sequences from 59 specimens of Arabian Verbascum were analysed. The phylogenetic analysis of one nuclear (ITS) and three chloroplastic (rbcL, matK, trnL) markers confirmed the monophyly of Verbascum, including the genus Rhabdotosperma. In addition to presenting novel phylogenetic relationships among the different Verbascum species in the Arabian Peninsula, our study reduced the species count of Arabian Verbascum to 16. Moreover, the phylogenetic analysis strongly supports the reinstatement of the genus Rhabdotosperma into Verbascum based on the Bayesian and maximum parsimony analyses.

Original language	English
Journal	Taxon
Early online date	6 Mar 2024
DOIs	https://doi.org/10.1002/tax.13156
Publication status	E-pub ahead of print - 6 Mar 2024

Bibliographical note

ACKNOWLEDGEMENTS
We are grateful to the curators and staff of Royal Botanical Gardens, Kew and Royal Botanical Garden Edinburgh for providing access to herbarium specimens and the samples for DNA barcoding. Thanks also to acknowledge Al-Baha University for financial support.

Keywords

Verbascum
Arabian Peninsula
species status
phylogeny
Rhabdotosperma

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title = "DNA barcoding of the genus Verbascum (Scrophulariaceae) in the Arabian Peninsula",

abstract = "Verbascum and Rhabdotosperma are members of the family Scrophulariaceae. The first genus comprises approximately 360 species from almost all parts of the world, while the second contains a total of 8 species from tropical Africa and the Arabian Peninsula. Since 1977, the relationships between Verbascum and Rhabdotosperma continue to be contested. The present study aims to present the phylogenetic relationships and status among Verbascum species in the Arabian Peninsula. For phylogenetic analyses, maximum parsimony and Bayesian inference were performed. In total, 236 DNA sequences from 59 specimens of Arabian Verbascum were analysed. The phylogenetic analysis of one nuclear (ITS) and three chloroplastic (rbcL, matK, trnL) markers confirmed the monophyly of Verbascum, including the genus Rhabdotosperma. In addition to presenting novel phylogenetic relationships among the different Verbascum species in the Arabian Peninsula, our study reduced the species count of Arabian Verbascum to 16. Moreover, the phylogenetic analysis strongly supports the reinstatement of the genus Rhabdotosperma into Verbascum based on the Bayesian and maximum parsimony analyses.",

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author = "Alzahrani, {Ali Mohammed} and Brehm, {Joana Magos} and Ghazanfar, {Shahina A.} and Nigel Maxted",

note = "ACKNOWLEDGEMENTS We are grateful to the curators and staff of Royal Botanical Gardens, Kew and Royal Botanical Garden Edinburgh for providing access to herbarium specimens and the samples for DNA barcoding. Thanks also to acknowledge Al-Baha University for financial support.",

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journal = "Taxon",

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T1 - DNA barcoding of the genus Verbascum (Scrophulariaceae) in the Arabian Peninsula

AU - Alzahrani, Ali Mohammed

AU - Brehm, Joana Magos

AU - Ghazanfar, Shahina A.

AU - Maxted, Nigel

N1 - ACKNOWLEDGEMENTS We are grateful to the curators and staff of Royal Botanical Gardens, Kew and Royal Botanical Garden Edinburgh for providing access to herbarium specimens and the samples for DNA barcoding. Thanks also to acknowledge Al-Baha University for financial support.

PY - 2024/3/6

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N2 - Verbascum and Rhabdotosperma are members of the family Scrophulariaceae. The first genus comprises approximately 360 species from almost all parts of the world, while the second contains a total of 8 species from tropical Africa and the Arabian Peninsula. Since 1977, the relationships between Verbascum and Rhabdotosperma continue to be contested. The present study aims to present the phylogenetic relationships and status among Verbascum species in the Arabian Peninsula. For phylogenetic analyses, maximum parsimony and Bayesian inference were performed. In total, 236 DNA sequences from 59 specimens of Arabian Verbascum were analysed. The phylogenetic analysis of one nuclear (ITS) and three chloroplastic (rbcL, matK, trnL) markers confirmed the monophyly of Verbascum, including the genus Rhabdotosperma. In addition to presenting novel phylogenetic relationships among the different Verbascum species in the Arabian Peninsula, our study reduced the species count of Arabian Verbascum to 16. Moreover, the phylogenetic analysis strongly supports the reinstatement of the genus Rhabdotosperma into Verbascum based on the Bayesian and maximum parsimony analyses.

AB - Verbascum and Rhabdotosperma are members of the family Scrophulariaceae. The first genus comprises approximately 360 species from almost all parts of the world, while the second contains a total of 8 species from tropical Africa and the Arabian Peninsula. Since 1977, the relationships between Verbascum and Rhabdotosperma continue to be contested. The present study aims to present the phylogenetic relationships and status among Verbascum species in the Arabian Peninsula. For phylogenetic analyses, maximum parsimony and Bayesian inference were performed. In total, 236 DNA sequences from 59 specimens of Arabian Verbascum were analysed. The phylogenetic analysis of one nuclear (ITS) and three chloroplastic (rbcL, matK, trnL) markers confirmed the monophyly of Verbascum, including the genus Rhabdotosperma. In addition to presenting novel phylogenetic relationships among the different Verbascum species in the Arabian Peninsula, our study reduced the species count of Arabian Verbascum to 16. Moreover, the phylogenetic analysis strongly supports the reinstatement of the genus Rhabdotosperma into Verbascum based on the Bayesian and maximum parsimony analyses.

KW - Verbascum

KW - Arabian Peninsula

KW - species status

KW - phylogeny

KW - Rhabdotosperma

U2 - 10.1002/tax.13156

DO - 10.1002/tax.13156

M3 - Article

SN - 0040-0262

JO - Taxon

JF - Taxon

ER -

DNA barcoding of the genus Verbascum (Scrophulariaceae) in the Arabian Peninsula

Abstract

Bibliographical note

Keywords

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