Abstract
Ionic substitutions have been proposed as a tool to control the functional behavior of synthetic hydroxyapatite (HA), particularly for Bone Tissue Engineering applications. The effect of simultaneous substitution of different levels of carbonate (CO3 ) and silicon (Si) ions in the HA lattice was investigated. Furthermore, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on multi-substituted HA (SiCHA) to determine if biomimetic chemical compositions were osteoconductive. Of the four different compositions investigates, SiCHA-1 (0.58 wt % Si) and SiCHA-2 (0.45 wt % Si) showed missing bands for CO3 and Si using FTIR analysis, indicating competition for occupation of the phosphate site in the HA lattice; 500°C was considered the most favorable calcination temperature as: (i) the powders produced possessed a similar amount of CO3 (2-8 wt %) and Si (<1.0 wt %) as present in native bone; and (ii) there was a minimal loss of CO3 and Si from the HA structure to the surroundings during calcination. Higher Si content in SiCHA-1 led to lower cell viability and at most hindered proliferation, but no toxicity effect occurred. While, lower Si content in SiCHA-2 showed the highest ALP/DNA ratio after 21 days culture with hMSCs, indicating that the powder may stimulate osteogenic behavior to a greater extent than other powders. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1775-1785, 2017.
Original language | English |
---|---|
Pages (from-to) | 1775-1785 |
Number of pages | 11 |
Journal | Journal of Biomedical Materials Research Part A |
Volume | 105 |
Issue number | 6 |
Early online date | 15 Feb 2017 |
DOIs | |
Publication status | Published - Jun 2017 |
Keywords
- Adult
- Bone Substitutes/chemistry
- Bone and Bones/cytology
- Carbonates/chemistry
- Cell Survival
- Cells, Cultured
- Durapatite/chemistry
- Humans
- Male
- Materials Testing
- Mesenchymal Stromal Cells/cytology
- Osteoblasts/cytology
- Osteogenesis
- Powders
- Silicon/chemistry
- Tissue Engineering/methods
- X-Ray Diffraction
- Young Adult