Development of multisubstituted hydroxyapatite nanopowders as biomedical materials for bone tissue engineering applications

Yanny M Baba Ismail, Ian Wimpenny, Oana Bretcanu, Kenneth Dalgarno, Alicia J El Haj

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Ionic substitutions have been proposed as a tool to control the functional behavior of synthetic hydroxyapatite (HA), particularly for Bone Tissue Engineering applications. The effect of simultaneous substitution of different levels of carbonate (CO3 ) and silicon (Si) ions in the HA lattice was investigated. Furthermore, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on multi-substituted HA (SiCHA) to determine if biomimetic chemical compositions were osteoconductive. Of the four different compositions investigates, SiCHA-1 (0.58 wt % Si) and SiCHA-2 (0.45 wt % Si) showed missing bands for CO3 and Si using FTIR analysis, indicating competition for occupation of the phosphate site in the HA lattice; 500°C was considered the most favorable calcination temperature as: (i) the powders produced possessed a similar amount of CO3 (2-8 wt %) and Si (<1.0 wt %) as present in native bone; and (ii) there was a minimal loss of CO3 and Si from the HA structure to the surroundings during calcination. Higher Si content in SiCHA-1 led to lower cell viability and at most hindered proliferation, but no toxicity effect occurred. While, lower Si content in SiCHA-2 showed the highest ALP/DNA ratio after 21 days culture with hMSCs, indicating that the powder may stimulate osteogenic behavior to a greater extent than other powders. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1775-1785, 2017.

Original languageEnglish
Pages (from-to)1775-1785
Number of pages11
JournalJournal of Biomedical Materials Research Part A
Issue number6
Early online date15 Feb 2017
Publication statusPublished - Jun 2017


  • Adult
  • Bone Substitutes/chemistry
  • Bone and Bones/cytology
  • Carbonates/chemistry
  • Cell Survival
  • Cells, Cultured
  • Durapatite/chemistry
  • Humans
  • Male
  • Materials Testing
  • Mesenchymal Stromal Cells/cytology
  • Osteoblasts/cytology
  • Osteogenesis
  • Powders
  • Silicon/chemistry
  • Tissue Engineering/methods
  • X-Ray Diffraction
  • Young Adult


Dive into the research topics of 'Development of multisubstituted hydroxyapatite nanopowders as biomedical materials for bone tissue engineering applications'. Together they form a unique fingerprint.

Cite this