Determining the LIF-sensitive period for implantation using a LIF-receptor antagonist

L Mohamet, John Heath, SJ Kimber

Research output: Contribution to journalArticle

18 Citations (Scopus)


Uteri of Lif null mice do not support embryo implantation. Since deletion of some genes often prevents the survival of null mice to adulthood, we have used a proven inhibitor of leukaemia inhibitory factor (LIF) signalling to identify the precise window of time during which LIF is required in vivo, and assessed the cellular expression of several LIF-associated targets. On day 4 of pregnancy, mice were injected with hLIF-05 (inhibitor) into the uterine lumen, with corresponding volumes of PBS (vehicle) injected into the contralateral horn. On days 5 and 6, the number of implantation sites was recorded and the uteri processed for immunohistochemistry. Blockade of LIF on day 4 reduced embryo implantation by 50% (P
Original languageEnglish
Pages (from-to)827-836
Number of pages10
Issue number5
Publication statusPublished - 1 Nov 2009


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