Cyclops-independent floor plate differentiation in zebrafish embryos

Stéphanie Albert; Ferenc Müller; Nadine Fischer; Dominique Biellmann; Carl Neumann; Patrick Blader; Uwe Strähle

doi:10.1002/dvdy.10211

Cyclops-independent floor plate differentiation in zebrafish embryos

Stéphanie Albert, Ferenc Müller, Nadine Fischer, Dominique Biellmann, Carl Neumann, Patrick Blader, Uwe Strähle

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

In zebrafish, development of the ventral neural tube depends on the Nodal-related signal Cyclops (Cyc). One-day-old cyc mutant embryos lack the medial floor plate (MFP). We show here that cells expressing MFP marker genes differentiate gradually in cyc mutant embryos in a delayed manner during the second day of development. This late differentiation is restricted to the hindbrain and spinal cord and depends on an intact Hedgehog (Hh) signalling pathway. Cells expressing MFP marker genes in cyc mutant embryos appear to be derived from lateral floor plate (LFP) cells as they coexpress LFP and MFP marker genes. This finding suggests that the correct temporal development of the MFP is required for the distinction of LFP and MFP cells in wild-type embryos.

Original language	English
Pages (from-to)	59-66
Number of pages	8
Journal	Developmental Dynamics
Volume	226
Issue number	1
DOIs	https://doi.org/10.1002/dvdy.10211
Publication status	Published - Jan 2003

Keywords

Alleles
Animals
Cell Differentiation
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Intracellular Signaling Peptides and Proteins
Microscopy, Video
Mutation
Neural Crest
Rhombencephalon
Signal Transduction
Spinal Cord
Time Factors
Transfection
Transforming Growth Factor beta
Transgenes
Zebrafish
Zebrafish Proteins

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10.1002/dvdy.10211

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title = "Cyclops-independent floor plate differentiation in zebrafish embryos",

abstract = "In zebrafish, development of the ventral neural tube depends on the Nodal-related signal Cyclops (Cyc). One-day-old cyc mutant embryos lack the medial floor plate (MFP). We show here that cells expressing MFP marker genes differentiate gradually in cyc mutant embryos in a delayed manner during the second day of development. This late differentiation is restricted to the hindbrain and spinal cord and depends on an intact Hedgehog (Hh) signalling pathway. Cells expressing MFP marker genes in cyc mutant embryos appear to be derived from lateral floor plate (LFP) cells as they coexpress LFP and MFP marker genes. This finding suggests that the correct temporal development of the MFP is required for the distinction of LFP and MFP cells in wild-type embryos.",

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author = "St{\'e}phanie Albert and Ferenc M{\"u}ller and Nadine Fischer and Dominique Biellmann and Carl Neumann and Patrick Blader and Uwe Str{\"a}hle",

year = "2003",

month = jan,

doi = "10.1002/dvdy.10211",

language = "English",

volume = "226",

pages = "59--66",

journal = "Developmental Dynamics",

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publisher = "John Wiley & Sons",

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TY - JOUR

T1 - Cyclops-independent floor plate differentiation in zebrafish embryos

AU - Albert, Stéphanie

AU - Müller, Ferenc

AU - Fischer, Nadine

AU - Biellmann, Dominique

AU - Neumann, Carl

AU - Blader, Patrick

AU - Strähle, Uwe

PY - 2003/1

Y1 - 2003/1

N2 - In zebrafish, development of the ventral neural tube depends on the Nodal-related signal Cyclops (Cyc). One-day-old cyc mutant embryos lack the medial floor plate (MFP). We show here that cells expressing MFP marker genes differentiate gradually in cyc mutant embryos in a delayed manner during the second day of development. This late differentiation is restricted to the hindbrain and spinal cord and depends on an intact Hedgehog (Hh) signalling pathway. Cells expressing MFP marker genes in cyc mutant embryos appear to be derived from lateral floor plate (LFP) cells as they coexpress LFP and MFP marker genes. This finding suggests that the correct temporal development of the MFP is required for the distinction of LFP and MFP cells in wild-type embryos.

AB - In zebrafish, development of the ventral neural tube depends on the Nodal-related signal Cyclops (Cyc). One-day-old cyc mutant embryos lack the medial floor plate (MFP). We show here that cells expressing MFP marker genes differentiate gradually in cyc mutant embryos in a delayed manner during the second day of development. This late differentiation is restricted to the hindbrain and spinal cord and depends on an intact Hedgehog (Hh) signalling pathway. Cells expressing MFP marker genes in cyc mutant embryos appear to be derived from lateral floor plate (LFP) cells as they coexpress LFP and MFP marker genes. This finding suggests that the correct temporal development of the MFP is required for the distinction of LFP and MFP cells in wild-type embryos.

KW - Alleles

KW - Animals

KW - Cell Differentiation

KW - Embryo, Nonmammalian

KW - Gene Expression Regulation, Developmental

KW - Intracellular Signaling Peptides and Proteins

KW - Microscopy, Video

KW - Mutation

KW - Neural Crest

KW - Rhombencephalon

KW - Signal Transduction

KW - Spinal Cord

KW - Time Factors

KW - Transfection

KW - Transforming Growth Factor beta

KW - Transgenes

KW - Zebrafish

KW - Zebrafish Proteins

U2 - 10.1002/dvdy.10211

DO - 10.1002/dvdy.10211

M3 - Article

C2 - 12508225

SN - 1058-8388

VL - 226

SP - 59

EP - 66

JO - Developmental Dynamics

JF - Developmental Dynamics

IS - 1

ER -

Cyclops-independent floor plate differentiation in zebrafish embryos

Abstract

Keywords

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