Abstract
In vitro induced Foxp3+ T regulatory (iTreg) cells form a novel and promising target for therapeutic tolerance induction. However, the potential of these cells as a target for the treatment of various immune diseases, as well as the factors involved in their development and function, remain debated. Here, we demonstrate in a myelin basic protein (MBP)-specific murine model of CNS autoimmune disease that adoptive transfer of antigen-specific iTreg cells ameliorates disease progression. Moreover, we show that the co-stimulatory molecule CTLA-4 mediates in vitro differentiation of iTreg cells. Finally, we demonstrate that the secreted, immunosuppressive cytokine IL-10 controls the ability of antigen-specific iTreg cells to suppress autoimmune disease. Overall, we conclude that antigen-specific iTreg cells, which depend on various immune regulatory molecules for their differentiation and function, represent a major target for effective immunotherapy of autoimmune disease.
Original language | English |
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Article number | e108023 |
Pages (from-to) | 1-8 |
Number of pages | 8 |
Journal | PLoS ONE |
Volume | 9 |
Issue number | 9 |
DOIs | |
Publication status | Published - 19 Sept 2014 |
Keywords
- Animals
- CTLA-4 Antigen
- Cell Differentiation
- Encephalomyelitis
- Flow Cytometry
- Forkhead Transcription Factors
- Interleukin-10
- Mice
- T-Lymphocytes, Regulatory
- Journal Article
- Research Support, Non-U.S. Gov't