Concentration and fate of histatins and acidic proline-rich proteins in the oral environment

M Campese, X Sun, Jos Bosch, FG Oppenheim, EJ Helmerhorst

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58 Citations (Scopus)


Saliva plays a critical role in the protection of oral hard and soft tissues and contains a multitude of constituents with well-characterized biological activities in vitro. Among these are histatins and acidic proline-rich proteins (PRPs). Nevertheless, few functional studies have recognized the structural instability of these proteins in the proteolytic environment of whole saliva. The aim of this investigation was to determine histatin and acidic PRP levels in parotid secretion (PS) and in whole saliva (WS) as well as to establish their susceptibility to proteolysis in these salivary fluids. Using cationic polyacrylamide gel electrophoresis and densitometric analysis the average total histatin concentration (histatin 1 + 3 + 5) in WS was determined to be 33.3 +/- 16.7 mu g/ml (n=22) and the average total acidic PRP concentration (PRP1/PIF-s + PRP3/PIF-f) was 427.9 +/- 123.3 mu g/ml (n=22). Histatin and acidic PRP concentrations in PS were 6 and 1.5 times higher than in WS (n=7), respectively. WS histatin and acidic PRP levels each correlated significantly with WS total protein concentrations (P
Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalArchives of Oral Biology
Issue number4
Publication statusPublished - 1 Apr 2009


  • Proteolysis
  • Histatin
  • Oral
  • Enzymes
  • Peptides
  • Saliva
  • Proline-rich proteins


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