TY - JOUR
T1 - Biosensors for detection of mercury in contaminated soils
AU - Brown, Nigel
AU - Bontidean, I
AU - Mortari, A
AU - Leth, S
AU - Karlson, U
AU - Larsen, MM
AU - Vrangonsveld, J
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Biosensors based on whole bacterial cells and on bacterial heavy metal binding protein were used to determine the mercury concentration in soil. The soil samples were collected in a vegetable garden accidentally contaminated with elemental mercury 25 years earlier. Bioavailable mercury was measured using different sensors: a protein-based biosensor, a whole bacterial cell based biosensor, and a plant sensor, i.e. morphological and biochemical responses in primary leaves and roots of bean seedlings grown in the mercury-contaminated soil. For comparison the total mercury concentration of the soil samples was determined by AAS. Whole bacterial cell and protein-based biosensors gave accurate responses proportional to the total amount of mercury in the soil samples. On the contrary, plant sensors were found to be less useful indicators of soil mercury contamination, as determined by plant biomass, mercury content of primary leaves and enzyme activities.
AB - Biosensors based on whole bacterial cells and on bacterial heavy metal binding protein were used to determine the mercury concentration in soil. The soil samples were collected in a vegetable garden accidentally contaminated with elemental mercury 25 years earlier. Bioavailable mercury was measured using different sensors: a protein-based biosensor, a whole bacterial cell based biosensor, and a plant sensor, i.e. morphological and biochemical responses in primary leaves and roots of bean seedlings grown in the mercury-contaminated soil. For comparison the total mercury concentration of the soil samples was determined by AAS. Whole bacterial cell and protein-based biosensors gave accurate responses proportional to the total amount of mercury in the soil samples. On the contrary, plant sensors were found to be less useful indicators of soil mercury contamination, as determined by plant biomass, mercury content of primary leaves and enzyme activities.
KW - capacitive biosensor
KW - plant sensor
KW - whole cell
KW - soil
KW - mercury
UR - http://www.scopus.com/inward/record.url?scp=3042598894&partnerID=8YFLogxK
U2 - 10.1016/j.envpol.2004.02.019
DO - 10.1016/j.envpol.2004.02.019
M3 - Article
C2 - 15234092
VL - 131
SP - 255
EP - 262
JO - Environmental Pollution
JF - Environmental Pollution
ER -