TY - JOUR
T1 - Bifidobacterium breve UCC2003 Induces a Distinct Global Transcriptomic Program in Neonatal Murine Intestinal Epithelial Cells
AU - Kiu, Raymond
AU - Treveil, Agatha
AU - Harnisch, Lukas C.
AU - Caim, Shabhonam
AU - Leclaire, Charlotte
AU - Sinderen, Douwe van
AU - Korcsmaros, Tamas
AU - Hall, Lindsay J.
PY - 2020/7/24
Y1 - 2020/7/24
N2 - The underlying health-driving mechanisms of Bifidobacterium during early life are not well understood, particularly how this microbiota member may modulate the intestinal barrier via programming of intestinal epithelial cells (IECs). We investi- gated the impact of Bifidobacterium breve UCC2003 on the transcriptome of neonatal murine IECs. Small IECs from two-week-old neonatal mice administered B. breve UCC2003 or PBS (control) were subjected to global RNA sequencing, and differentially expressed genes, pathways, and affected cell types were deter- mined. We observed extensive regulation of the IEC transcriptome with $4,000 genes significantly up-regulated, including key genes linked with epithelial bar- rier function. Enrichment of cell differentiation pathways was observed, along with an overrepresentation of stem cell marker genes, indicating an increase in the regenerative potential of the epithelial layer. In conclusion, B. breve UCC2003 plays a central role in driving intestinal epithelium homeostatic devel- opment during early life and suggests future avenues for next-stage clinical studies.
AB - The underlying health-driving mechanisms of Bifidobacterium during early life are not well understood, particularly how this microbiota member may modulate the intestinal barrier via programming of intestinal epithelial cells (IECs). We investi- gated the impact of Bifidobacterium breve UCC2003 on the transcriptome of neonatal murine IECs. Small IECs from two-week-old neonatal mice administered B. breve UCC2003 or PBS (control) were subjected to global RNA sequencing, and differentially expressed genes, pathways, and affected cell types were deter- mined. We observed extensive regulation of the IEC transcriptome with $4,000 genes significantly up-regulated, including key genes linked with epithelial bar- rier function. Enrichment of cell differentiation pathways was observed, along with an overrepresentation of stem cell marker genes, indicating an increase in the regenerative potential of the epithelial layer. In conclusion, B. breve UCC2003 plays a central role in driving intestinal epithelium homeostatic devel- opment during early life and suggests future avenues for next-stage clinical studies.
KW - Bifidobacterium
KW - Transcriptomics
KW - Epithelial Cells/microbiology
UR - https://doi.org/10.1016/j.isci.2020.101336
U2 - 10.1016/j.isci.2020.101336
DO - 10.1016/j.isci.2020.101336
M3 - Article
SN - 2589-0042
VL - 23
JO - iScience
JF - iScience
IS - 7
M1 - 101336
ER -