Antimicrobial properties of Pseudomonas strains producing the antibiotic mupirocin

Sandra Matthijs; Corinne Vander Wauven; Bertrand Cornu; Lumeng Ye; Pierre Cornelis; Christopher Thomas; Marc Ongena

doi:10.1016/j.resmic.2014.09.009

Antimicrobial properties of Pseudomonas strains producing the antibiotic mupirocin

Sandra Matthijs, Corinne Vander Wauven, Bertrand Cornu, Lumeng Ye, Pierre Cornelis, Christopher Thomas, Marc Ongena

Biosciences

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Abstract

Mupirocin is a polyketide antibiotic with broad antibacterial activity. It was isolated and characterized about 40 years ago from Pseudomonas fluorescens NCIMB 10586. To study the phylogenetic distribution of mupirocin producing strains in the genus Pseudomonas a large collection of Pseudomonas strains of worldwide origin, consisting of 117 Pseudomonas type strains and 461 strains isolated from different biological origins, was screened by PCR for the mmpD gene of the mupirocin gene cluster. Five mmpD+ strains from different geographic and biological origin were identified. They all produced mupirocin and were strongly antagonistic against Staphylococcus aureus. Phylogenetic analysis showed that mupirocin production is limited to a single species.

Inactivation of mupirocin production leads to complete loss of in vitro antagonism against S. aureus, except on certain iron-reduced media where the siderophore pyoverdine is responsible for the in vitro antagonism of a mupirocin-negative mutant. In addition to mupirocin some of the strains produced lipopeptides of the massetolide group. These lipopeptides do not play a role in the observed in vitro antagonism of the mupirocin producing strains against S. aureus.

Original language	English
Pages (from-to)	695-704
Journal	Research in Microbiology
Volume	165
Issue number	8
Early online date	7 Oct 2014
DOIs	https://doi.org/10.1016/j.resmic.2014.09.009
Publication status	Published - Oct 2014

Keywords

Mupirocin
Massetolide
Pyoverdine
Pseudomonas fluorescens NCIMB 10586
Pseudomonas sp. W2Aug9

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10.1016/j.resmic.2014.09.009

Thomas_Antimicrobial_properties_Pseudomonas_strains_Research_Microbiology_2014
NOTICE: this is the author’s version of a work that was accepted for publication. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published as S. Matthijs, C.V. Wauven, B. Cornu, Y. Lumeng, P. Cornelis, C.M. Thomas, M. Ongena, Antimicrobial properties of Pseudomonas strains producing the antibiotic mupirocin, Research in Microbiology (2014), doi: 10.1016/j.resmic.2014.09.009.
Accepted author manuscript, 1.06 MBLicence: Other (please specify with Rights Statement)

http://linkinghub.elsevier.com/retrieve/pii/S0923250814001892

Novel hybrid anti-MRSA antibiotics from manipulation of the mupirocin and thiomarinol biosynthetic pathways
Thomas, C. & Winn, P.
Biotechnology & Biological Sciences Research Council
1/10/11 → 31/12/14
Project: Research Councils

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title = "Antimicrobial properties of Pseudomonas strains producing the antibiotic mupirocin",

abstract = "Mupirocin is a polyketide antibiotic with broad antibacterial activity. It was isolated and characterized about 40 years ago from Pseudomonas fluorescens NCIMB 10586. To study the phylogenetic distribution of mupirocin producing strains in the genus Pseudomonas a large collection of Pseudomonas strains of worldwide origin, consisting of 117 Pseudomonas type strains and 461 strains isolated from different biological origins, was screened by PCR for the mmpD gene of the mupirocin gene cluster. Five mmpD+ strains from different geographic and biological origin were identified. They all produced mupirocin and were strongly antagonistic against Staphylococcus aureus. Phylogenetic analysis showed that mupirocin production is limited to a single species.Inactivation of mupirocin production leads to complete loss of in vitro antagonism against S. aureus, except on certain iron-reduced media where the siderophore pyoverdine is responsible for the in vitro antagonism of a mupirocin-negative mutant. In addition to mupirocin some of the strains produced lipopeptides of the massetolide group. These lipopeptides do not play a role in the observed in vitro antagonism of the mupirocin producing strains against S. aureus.",

keywords = "Mupirocin, Massetolide, Pyoverdine, Pseudomonas fluorescens NCIMB 10586, Pseudomonas sp. W2Aug9",

author = "Sandra Matthijs and {Vander Wauven}, Corinne and Bertrand Cornu and Lumeng Ye and Pierre Cornelis and Christopher Thomas and Marc Ongena",

year = "2014",

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AU - Matthijs, Sandra

AU - Vander Wauven, Corinne

AU - Cornu, Bertrand

AU - Ye, Lumeng

AU - Cornelis, Pierre

AU - Thomas, Christopher

AU - Ongena, Marc

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Y1 - 2014/10

N2 - Mupirocin is a polyketide antibiotic with broad antibacterial activity. It was isolated and characterized about 40 years ago from Pseudomonas fluorescens NCIMB 10586. To study the phylogenetic distribution of mupirocin producing strains in the genus Pseudomonas a large collection of Pseudomonas strains of worldwide origin, consisting of 117 Pseudomonas type strains and 461 strains isolated from different biological origins, was screened by PCR for the mmpD gene of the mupirocin gene cluster. Five mmpD+ strains from different geographic and biological origin were identified. They all produced mupirocin and were strongly antagonistic against Staphylococcus aureus. Phylogenetic analysis showed that mupirocin production is limited to a single species.Inactivation of mupirocin production leads to complete loss of in vitro antagonism against S. aureus, except on certain iron-reduced media where the siderophore pyoverdine is responsible for the in vitro antagonism of a mupirocin-negative mutant. In addition to mupirocin some of the strains produced lipopeptides of the massetolide group. These lipopeptides do not play a role in the observed in vitro antagonism of the mupirocin producing strains against S. aureus.

AB - Mupirocin is a polyketide antibiotic with broad antibacterial activity. It was isolated and characterized about 40 years ago from Pseudomonas fluorescens NCIMB 10586. To study the phylogenetic distribution of mupirocin producing strains in the genus Pseudomonas a large collection of Pseudomonas strains of worldwide origin, consisting of 117 Pseudomonas type strains and 461 strains isolated from different biological origins, was screened by PCR for the mmpD gene of the mupirocin gene cluster. Five mmpD+ strains from different geographic and biological origin were identified. They all produced mupirocin and were strongly antagonistic against Staphylococcus aureus. Phylogenetic analysis showed that mupirocin production is limited to a single species.Inactivation of mupirocin production leads to complete loss of in vitro antagonism against S. aureus, except on certain iron-reduced media where the siderophore pyoverdine is responsible for the in vitro antagonism of a mupirocin-negative mutant. In addition to mupirocin some of the strains produced lipopeptides of the massetolide group. These lipopeptides do not play a role in the observed in vitro antagonism of the mupirocin producing strains against S. aureus.

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KW - Pyoverdine

KW - Pseudomonas fluorescens NCIMB 10586

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DO - 10.1016/j.resmic.2014.09.009

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SP - 695

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JO - Research in Microbiology

JF - Research in Microbiology

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ER -

Antimicrobial properties of Pseudomonas strains producing the antibiotic mupirocin

Abstract

Keywords

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Novel hybrid anti-MRSA antibiotics from manipulation of the mupirocin and thiomarinol biosynthetic pathways

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