An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Sandra Hasenkamp; Eleanor H. Wong; Paul Horrocks

doi:10.1186/1475-2875-11-42

An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Sandra Hasenkamp, Eleanor H. Wong, Paul Horrocks^*

^*Corresponding author for this work

Biosciences

Research output: Contribution to journal › Article › peer-review

Abstract

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

Original language	English
Article number	42
Journal	Malaria Journal
Volume	11
DOIs	https://doi.org/10.1186/1475-2875-11-42
Publication status	Published - 2012

Bibliographical note

Funding Information:
This work was supported by funds from the Royal Society to SH and PH and the BBSRC through a New Investigator Award to PH. We would like to thank Alister Craig for critically reading the manuscript.

Keywords

Luciferase
pcna
Promoter function
Single-step lysis
Stage-specific

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/1475-2875-11-42

Cite this

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title = "An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum",

abstract = "This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.",

keywords = "Luciferase, pcna, Promoter function, Single-step lysis, Stage-specific",

author = "Sandra Hasenkamp and Wong, {Eleanor H.} and Paul Horrocks",

note = "Funding Information: This work was supported by funds from the Royal Society to SH and PH and the BBSRC through a New Investigator Award to PH. We would like to thank Alister Craig for critically reading the manuscript.",

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AU - Hasenkamp, Sandra

AU - Wong, Eleanor H.

AU - Horrocks, Paul

N1 - Funding Information: This work was supported by funds from the Royal Society to SH and PH and the BBSRC through a New Investigator Award to PH. We would like to thank Alister Craig for critically reading the manuscript.

PY - 2012

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N2 - This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

AB - This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

KW - Luciferase

KW - pcna

KW - Promoter function

KW - Single-step lysis

KW - Stage-specific

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JO - Malaria Journal

JF - Malaria Journal

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ER -

An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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Cite this