Abstract
This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.
Original language | English |
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Article number | 42 |
Journal | Malaria Journal |
Volume | 11 |
DOIs | |
Publication status | Published - 2012 |
Bibliographical note
Funding Information:This work was supported by funds from the Royal Society to SH and PH and the BBSRC through a New Investigator Award to PH. We would like to thank Alister Craig for critically reading the manuscript.
Keywords
- Luciferase
- pcna
- Promoter function
- Single-step lysis
- Stage-specific
ASJC Scopus subject areas
- Parasitology
- Infectious Diseases