An improved conjugation method for Pseudomonas syringae

Robert Jackson, Michelle Hulin, Helen Neale, Richard Harrison, John Mansfield, Dawn Arnold

Research output: Contribution to journalArticlepeer-review


In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.
Original languageEnglish
Article number106025
JournalJournal of Microbiological Methods
Publication statusPublished - Oct 2020

Bibliographical note

Funding Information:
We thank Adam Deutschbauer (Lawrence Berkeley National Laboratory, Berkeley, California, USA) for providing us with mariner vector pKMW3. This work was carried out under the Department for Environment, Food and Rural Affairs' Plant Health and Seeds Inspectorate license number 51049/202078/1. This research was funded by grants from the Biotechnology and Biological Sciences Research Council , United Kingdom (UWE grant number BB/P005705/1 and EMR grant number BB/P006272/1 ).

Publisher Copyright:
© 2020 Elsevier B.V.


  • Conjugation
  • Pseudomonas
  • TnSeq

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)


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