Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis

J Hill, P W Andrew, T J Mitchell

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.

Original languageEnglish
Pages (from-to)757-8
Number of pages2
JournalInfection and Immunity
Volume62
Issue number2
Publication statusPublished - Feb 1994

Keywords

  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins
  • Cloning, Molecular
  • Genes, Bacterial
  • Hemolysis
  • In Vitro Techniques
  • Mutagenesis
  • Point Mutation
  • Sheep
  • Streptococcus pneumoniae
  • Streptolysins
  • Virulence

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