Abstract
The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.
Original language | English |
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Pages (from-to) | 757-8 |
Number of pages | 2 |
Journal | Infection and Immunity |
Volume | 62 |
Issue number | 2 |
Publication status | Published - Feb 1994 |
Keywords
- Amino Acid Sequence
- Animals
- Bacterial Proteins
- Cloning, Molecular
- Genes, Bacterial
- Hemolysis
- In Vitro Techniques
- Mutagenesis
- Point Mutation
- Sheep
- Streptococcus pneumoniae
- Streptolysins
- Virulence