Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis

J Hill; P W Andrew; T J Mitchell

Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis

J Hill, P W Andrew, T J Mitchell

Microbiology and Infection

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.

Original language	English
Pages (from-to)	757-8
Number of pages	2
Journal	Infection and Immunity
Volume	62
Issue number	2
Publication status	Published - Feb 1994

Keywords

Amino Acid Sequence
Animals
Bacterial Proteins
Cloning, Molecular
Genes, Bacterial
Hemolysis
In Vitro Techniques
Mutagenesis
Point Mutation
Sheep
Streptococcus pneumoniae
Streptolysins
Virulence

Cite this

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title = "Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis",

abstract = "The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.",

keywords = "Amino Acid Sequence, Animals, Bacterial Proteins, Cloning, Molecular, Genes, Bacterial, Hemolysis, In Vitro Techniques, Mutagenesis, Point Mutation, Sheep, Streptococcus pneumoniae, Streptolysins, Virulence",

author = "J Hill and Andrew, {P W} and Mitchell, {T J}",

year = "1994",

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language = "English",

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pages = "757--8",

journal = "Infection and Immunity",

issn = "0019-9567",

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T1 - Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis

AU - Hill, J

AU - Andrew, P W

AU - Mitchell, T J

PY - 1994/2

Y1 - 1994/2

N2 - The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.

AB - The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.

KW - Amino Acid Sequence

KW - Animals

KW - Bacterial Proteins

KW - Cloning, Molecular

KW - Genes, Bacterial

KW - Hemolysis

KW - In Vitro Techniques

KW - Mutagenesis

KW - Point Mutation

KW - Sheep

KW - Streptococcus pneumoniae

KW - Streptolysins

KW - Virulence

M3 - Article

C2 - 8300240

SN - 0019-9567

VL - 62

SP - 757

EP - 758

JO - Infection and Immunity

JF - Infection and Immunity

IS - 2

ER -

Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis

Abstract

Keywords

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