A Versatile Elastin-Like Carrier for Bioactive Antimicrobial Peptide Production and Delivery

Laura ColominaAlfaro; Paola Sist; Silvia Marchesan; Ranieri Urbani; Artemis Stamboulis; Antonella Bandiera

doi:10.1002/mabi.202300236

A Versatile Elastin-Like Carrier for Bioactive Antimicrobial Peptide Production and Delivery

Laura ColominaAlfaro, Paola Sist, Silvia Marchesan, Ranieri Urbani, Artemis Stamboulis, Antonella Bandiera^*

^*Corresponding author for this work

Metallurgy and Materials

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Abstract

Elastin‐like polypeptides are biotechnological protein and peptide carriers that offer a vast scope of applicability. This work aims to build a model for the expression of antimicrobial peptides (AMPs) by genetically engineering the Human Elastin‐like Polypeptide platform developed in the lab. The well‐characterized AMP indolicidin is selected as an example of an antimicrobial domain for the recombinant fusion at the C‐terminus of the carrier. The fusion construct has been designed to allow the release of the antimicrobial domain. The expression product has been purified and its physicochemical and antimicrobial properties has been characterized. Taking advantage of the self‐assembling and matrix‐forming properties of the recombinant biopolymer, the materials that are obtained have been evaluated for antimicrobial activity toward bacterial‐strain models. This approach represents a cost‐effective strategy for the production of smart components and materials endowed with antimicrobial capacity triggered by external stimuli.

Original language	English
Article number	e2300236
Journal	Macromolecular Bioscience
Early online date	12 Sept 2023
DOIs	https://doi.org/10.1002/mabi.202300236
Publication status	E-pub ahead of print - 12 Sept 2023

Bibliographical note

Acknowledgments:
The authors were grateful to Dr. Lucia Corich and Prof. Cristina Lagatolla for providing the bacterial strains used in this study, Prof. Sabina Passamonti for her helpful support, and Dr. Marco Stebel, Dr. Davide Porrelli, and Dr. Fabio Hollan for technical assistance. This work was supported by the Horizon 2020 Innovative Training Network AIMed project under the Marie Skłodowska-Curie, grant agreement No 861138, and the Horizon Europe STOP – Surface Transfer of Pathogens project, grant agreement No 101057961.

Keywords

antimicrobial peptide
recombinant biopolymers
smart materials
hydrogel matrix

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10.1002/mabi.202300236Licence: Creative Commons: Attribution (CC BY)

ColominaL2023VersatileFinal published version, 1.18 MBLicence: Creative Commons: Attribution (CC BY)

STOP - Surface Transfer of Pathogens
Stamboulis, A. & Dong, H.
UKRI Horizon Europe Underwriting â€“ Innovate UK
1/09/22 → 31/08/26
Project: Research
AIMed: Antimicrobial Integrated Methodologies for orthopaedic applications
Stamboulis, A.
European Commission, European Commission - Management Costs
1/01/20 → 31/12/24
Project: Research

Cite this

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title = "A Versatile Elastin-Like Carrier for Bioactive Antimicrobial Peptide Production and Delivery",

abstract = "Elastin‐like polypeptides are biotechnological protein and peptide carriers that offer a vast scope of applicability. This work aims to build a model for the expression of antimicrobial peptides (AMPs) by genetically engineering the Human Elastin‐like Polypeptide platform developed in the lab. The well‐characterized AMP indolicidin is selected as an example of an antimicrobial domain for the recombinant fusion at the C‐terminus of the carrier. The fusion construct has been designed to allow the release of the antimicrobial domain. The expression product has been purified and its physicochemical and antimicrobial properties has been characterized. Taking advantage of the self‐assembling and matrix‐forming properties of the recombinant biopolymer, the materials that are obtained have been evaluated for antimicrobial activity toward bacterial‐strain models. This approach represents a cost‐effective strategy for the production of smart components and materials endowed with antimicrobial capacity triggered by external stimuli.",

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AU - Stamboulis, Artemis

AU - Bandiera, Antonella

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AB - Elastin‐like polypeptides are biotechnological protein and peptide carriers that offer a vast scope of applicability. This work aims to build a model for the expression of antimicrobial peptides (AMPs) by genetically engineering the Human Elastin‐like Polypeptide platform developed in the lab. The well‐characterized AMP indolicidin is selected as an example of an antimicrobial domain for the recombinant fusion at the C‐terminus of the carrier. The fusion construct has been designed to allow the release of the antimicrobial domain. The expression product has been purified and its physicochemical and antimicrobial properties has been characterized. Taking advantage of the self‐assembling and matrix‐forming properties of the recombinant biopolymer, the materials that are obtained have been evaluated for antimicrobial activity toward bacterial‐strain models. This approach represents a cost‐effective strategy for the production of smart components and materials endowed with antimicrobial capacity triggered by external stimuli.

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A Versatile Elastin-Like Carrier for Bioactive Antimicrobial Peptide Production and Delivery

Abstract

Bibliographical note

Keywords

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Projects

STOP - Surface Transfer of Pathogens

AIMed: Antimicrobial Integrated Methodologies for orthopaedic applications

Cite this